A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts
文献类型：期刊论文
| 作者 | Ning, Dianhua1,2; He, Changtian1,2; Liu, Zhengjie1,2; Liu, Cui1,2; Wu, Qilong1,2; Zhao, TingTing1,3; Liu, Renyong1,2,3 |
| 刊名 | ANALYST
 |
| 出版日期 | 2017-05-21 |
| 卷号 | 142期号:10页码:1697-1702 |
| DOI | 10.1039/c7an00150a |
| 文献子类 | Article |
| 英文摘要 | Human telomerase RNA (hTR), which is one component of telomerase, was deemed to be a biomarker to monitor tumor cells due to its different expression levels in tumor cells and normal somatic cells. Thus far, plentiful fluorescent probes have been designed to investigate nucleic acids. However, most of them are limited since they are time-consuming, require professional operators and even result in false positive signals in the cellular environment. Herein, we report a dual-colored ratiometric-fluorescent oligonucleotide probe to achieve the reliable detection of human telomerase RNA in cell extracts. The probe is constructed using a dual-labeled fluorescent oligonucleotide hybridized with target-complemented Dabcyl-labeled oligonucleotide. In the presence of the target, the dual-labeled fluorescent oligonucleotide translates into a hairpin structure, which leads to the generation of the fluorescence resonance energy transfer (FRET) phenomenon under UV excitation. Compared to conventional methods, this strategy could effectively avoid false positive signals, and it not only possesses the advantages of simplicity and high specificity but also has the merits of signal stability and distinguishable color variation. Moreover, the quantitative assay of hTR would have a far-reaching impact on the telomerase mechanism and even tumor diagnosis research. |
| WOS关键词 | RESONANCE ENERGY-TRANSFER ; HYBRIDIZATION CHAIN-REACTION ; MESSENGER-RNA ; QUANTUM DOTS ; POLYACRYLAMIDE-GELS ; GRAPHENE OXIDE ; FRET ; DNA ; HTR ; EXPRESSION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000401526100007 |
| 资助机构 | National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/31863]  |
| 专题 | 合肥物质科学研究院_中科院合肥智能机械研究所 |
| 作者单位 | 1.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ning, Dianhua,He, Changtian,Liu, Zhengjie,et al. A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts[J]. ANALYST,2017,142(10):1697-1702. |
| APA | Ning, Dianhua.,He, Changtian.,Liu, Zhengjie.,Liu, Cui.,Wu, Qilong.,...&Liu, Renyong.(2017).A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts.ANALYST,142(10),1697-1702. |
| MLA | Ning, Dianhua,et al."A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts".ANALYST 142.10(2017):1697-1702. |
入库方式： OAI收割
来源：合肥物质科学研究院
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