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  2. 合肥物质科学研究院
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  4. 中科院合肥智能机械研究所
Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe

文献类型：期刊论文

作者Lei, Kepeng1,2; Sun, Mingtai1; Du, Libo5; Zhang, Xiaojie5; Yu, Huan1; Wang, Suhua1,2,3,4; Hayat, Tasawar3; Alsaedi, Ahmed3
刊名TALANTA
出版日期2017-08-01
卷号170期号:无页码:314-321
关键词Bodipy Cell Imaging Fluorescent Probe Hydroxyl Radical Sensitive And Selective
DOI10.1016/j.talanta.2017.04.004
文献子类Article
英文摘要The sensitive and selective fluorescence probe for hydroxyl radical analysis is of significance because hydroxyl radical plays key roles in many physiological and pathological processes. In this work, a novel organic fluorescence molecular probe OHP for hydroxyl radical is synthesized by a two-step route. The probe employs 4-bora-3a,4a-diaza-s-indacene (difluoroboron dipyrromethene, BODIPY) as the fluorophore and possesses relatively high fluorescence quantum yields (77.14%). Hydroxyl radical can rapidly react with the probe and quench the fluorescence in a good linear relationship (R-2=0.9967). The limit of detection is determined to be as low as 11 nM. In addition, it has been demonstrated that the probe has a good stability against pH and light illumination, low cytotoxicity and high biocompatibility. Cell culture experimental results show that the probe OHP is sensitive and selective for imaging and tracking endogenous hydroxyl radical in live cells.
WOS关键词TURN-ON DETECTION ; QUANTUM DOTS ; LIVING CELLS ; SELECTIVE DETECTION ; HYPOCHLOROUS ACID ; RATIOMETRIC FLUORESCENCE ; GOLD NANOCLUSTERS ; OXIDATIVE STRESS ; NITROGEN-DIOXIDE ; OXYGEN RADICALS
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000402343200043
资助机构National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; National Natural Science Foundation of China(21475134 ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; Fundamental Research Funds for the Central Universities(2016ZZD06) ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 91439101 ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135) ; 21507135)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/31957]  
专题合肥物质科学研究院_中科院合肥智能机械研究所
作者单位1.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Dept Mat Sci & Engn, Hefei 230026, Anhui, Peoples R China
3.King Abdulaziz Univ, NAAM Res Grp, Fac Sci, Jeddah 21589, Saudi Arabia
4.North China Elect Power Univ, Sch Environm & Chem Engn, Beijing 102206, Peoples R China
5.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Lei, Kepeng,Sun, Mingtai,Du, Libo,et al. Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe[J]. TALANTA,2017,170(无):314-321.
APA Lei, Kepeng.,Sun, Mingtai.,Du, Libo.,Zhang, Xiaojie.,Yu, Huan.,...&Alsaedi, Ahmed.(2017).Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe.TALANTA,170(无),314-321.
MLA Lei, Kepeng,et al."Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe".TALANTA 170.无(2017):314-321.

入库方式： OAI收割

来源：合肥物质科学研究院

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