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Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium

文献类型：期刊论文

作者Tong CF(佟春芳); Zhang Y(章燕); Lv SQ(吕守芹); Li N(李宁); Gong YX(龚一心); Yang H; Feng SL; Du Y; Huang DD; Long M(龙勉)
刊名AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
出版日期2018-09-01
卷号315期号:3页码:C409-C421
ISSN号0363-6143
关键词brain ICAM-1 integrin kinetics liver
DOI10.1152/ajpcell.00083.2017
英文摘要Flowing polymorphonuclear neutrophils (PMNs) are forced to recruit toward inflamed tissue and adhere to vascular endothelial cells, which is primarily mediated by the binding of beta(2)-integrins to ICAM-1. This process is distinct among different organs such as liver and brain; however, the underlying kinetic and mechanical mechanisms regulating tissue-specific recruitment of PMNs remain unclear. Here, binding kinetics measurement showed that ICAM-1 on murine hepatic sinusoidal endothelial cells (LSECs) bound to lymphocyte function-associated antigen-1 (LFA-1) with higher on-and off-rates but lower effective affinity compared with macrophage-1 antigen (Mac-1), whereas ICAM-1 on cerebral endothelial cells (BMECs or bEnd. 3 cells) bound to LFA-1 with higher on-rates, similar off-rates, and higher effective affinity compared with Mac-1. Physiologically, free crawling tests of PMN onto LSEC, BMEC, or bEnd. 3 monolayers were consistent with those kinetics differences between two beta(2)-integrins interacting with hepatic sinusoid or cerebral endothelium. Numerical calculations and Monte Carlo simulations validated tissue-specific contributions of beta(2)-integrin-ICAM-1 kinetics to PMN crawling on hepatic sinusoid or cerebral endothelium. Thus, this work first quantified the biophysical regulation of PMN adhesion in hepatic sinusoids compared with cerebral endothelium.
分类号二类
URL标识查看原文
WOS关键词BLOOD-BRAIN-BARRIER ; 2-DIMENSIONAL KINETICS ; NEUTROPHIL RECRUITMENT ; LEUKOCYTE RECRUITMENT ; LIVER METASTASIS ; INFLAMMATION ; SELECTIN ; MOTILITY ; MICROPIPETTE ; MECHANICS
WOS研究方向Cell Biology ; Physiology
语种英语
WOS记录号WOS:000444021100014
资助机构National Natural Science Foundation of China [31230027, 91642203, 31661143044] ; Chinese Academy of Sciences Strategic Priority Research Program [XDB22040101] ; Frontier Science Key Project [QYZDJ-SSW-JSC018] ; National Key Research and Development Program of China [2016YFA0501601]
源URL[http://dspace.imech.ac.cn/handle/311007/77952]  
专题力学研究所_国家微重力实验室
作者单位1.[Tong, Chun-Fang
2.Zhang, Yan
3.Lu, Shou-Qin
4.Li, Ning
5.Gong, Yi-Xin
6.Yang, Hao
7.Feng, Shi-Liang
8.Du, Yu
9.Huang, Dan-Dan
10.Chinese Acad Sci, Natl Micrograv Lab, Ctr Biomech & Bioengn, Key Lab Micrograv, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Tong CF,Zhang Y,Lv SQ,et al. Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2018,315(3):C409-C421.
APA 佟春芳.,章燕.,吕守芹.,李宁.,龚一心.,...&龙勉.(2018).Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,315(3),C409-C421.
MLA 佟春芳,et al."Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 315.3(2018):C409-C421.

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来源：力学研究所

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