Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway
文献类型：期刊论文
| 作者 | Zhao, Xiaoying1,3,4; Mei, Lijuan2; Pei, Jinjin2,5; Liu, Zenggen2 ; Shao, Yun2; Tao, Yanduo2; Zhang, Xiaoling1,3,4; Jiang, Lei2
|
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2017-11-08 |
| 卷号 | 65期号:44页码:9647-9654 |
| 关键词 | Osteoporosis Sophora Flower Sophoridine Osteoclast Differentiation Mapk Nfatc1 |
| DOI | 10.1021/acs.jafc.7b03666 |
| 英文摘要 | An imbalance in osteogenesis and osteoclastogenesis is a crucial pathological factor in the development of osteoporosis. Osteoclasts (OCs) play a pivotal role in osteoporosis, whose new therapy exploration has been focused on the suppression of OC formation. Sophoridine is found from the Chinese traditional food sophora flower to exhibit anti-osteoporosis capacity by screening. This study is focused on its anti -osteoporosis mechanism evaluation. The anti -osteoporosis effect of sophoridine, (15 mg kg(-1) body), was evaluated in ovariectomized (OVX) mice by monitoring changes in bone histomorphometry index, formation of osteoclasts from blood-derived mononuclear cells, and changes in the synthesis of pro-osteodastogenic cytokines. Signal pathways were investigated by QPCR, Western blot, and immunofluorescence. Sophoridine has a significant anti-osteoporosis effect in vivo, which can inhibit RANKL-induced OC formation, the appearance of OC-specific marker genes, and OC marker protein in vitro. Mechanistically, sophoridine dose- and time-dependently blocks the RANKL-induced OC formation and the activation of ERK and c-Fos as well as the induction and nucleus translocation of NFATc1. Sophora flower might be a useful alternative functional food in preventing or treating osteoporosis. |
| 语种 | 英语 |
| WOS记录号 | WOS:000415028700010 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/10360]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200025, Peoples R China 2.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Med, Xin Hua Hosp, Dept Orthoped Surg, Shanghai 200092, Peoples R China 5.Shaanxi Univ Technol, Shaanxi Key Lab Bioresources & Biol, Hanzhong 723001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xiaoying,Mei, Lijuan,Pei, Jinjin,et al. Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(44):9647-9654. |
| APA | Zhao, Xiaoying.,Mei, Lijuan.,Pei, Jinjin.,Liu, Zenggen.,Shao, Yun.,...&Jiang, Lei.(2017).Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(44),9647-9654. |
| MLA | Zhao, Xiaoying,et al."Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.44(2017):9647-9654. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。