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Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC

文献类型：期刊论文

作者Liu, Hongliu1; Gong, Chengcheng1; Liao, Zhixin1; Chen, Tao2; Li, Yulin2
刊名CHROMATOGRAPHIA
出版日期2017-11-01
卷号80期号:11页码:1651-1658
关键词Molecularly Imprinted Solid-phase Extraction Hsccc Clematis Akebioides Caffeic Acid Separation
DOI10.1007/s10337-017-3406-z
英文摘要An efficient strategy of using molecularly imprinted solid-phase extraction and high-speed counter-current chromatography (HSCCC) were established for separation and purification of three analogues from Clematis akebioides for the first time. The three analogues, including 26.30 mg caffeic acid, 84.20 mg p-coumaric acid, and 44.40 mg ferulic acid, were obtained from 200.00 mg of the crude sample. The molecularly imprinted polymer was prepared with caffeic acid as template molecule and acrylamide as functional monomers. The results showed that the caffeic acid and its analogues could be well enriched. Then, the extract was purified by HSCCC using a two-phase solvent system consisting of chloroform/acetonitrile/water/acetic acid (10:5:4:0.0004, v/v) and a continuous sample introduction system was adopted. The three organic acids were identified by H-1-NMR and C-13-NMR.
语种英语
WOS记录号WOS:000415157600006
源URL[http://ir.nwipb.ac.cn/handle/363003/10361]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,et al. Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC[J]. CHROMATOGRAPHIA,2017,80(11):1651-1658.
APA Liu, Hongliu,Gong, Chengcheng,Liao, Zhixin,Chen, Tao,&Li, Yulin.(2017).Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC.CHROMATOGRAPHIA,80(11),1651-1658.
MLA Liu, Hongliu,et al."Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC".CHROMATOGRAPHIA 80.11(2017):1651-1658.

入库方式： OAI收割

来源：西北高原生物研究所

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