Antioxidants screening in Limonium aureum by optimized on-line HPLC-DPPH assay
文献类型：期刊论文
| 作者 | Geng, Dandan1,2; Chi, Xiaofeng1; Dong, Qi1; Hu, Fengzu1 |
| 刊名 | INDUSTRIAL CROPS AND PRODUCTS
 |
| 出版日期 | 2015-05-01 |
| 卷号 | 67页码:492-497 |
| 关键词 | Limonium Aureum (Linn.) Antioxidant On-line Hplc-dpph Response Surface Methodology |
| DOI | 10.1016/j.indcrop.2015.01.063 |
| 英文摘要 | Limonium aureum (Linn.) is a traditional Chinese medicine with strong antioxidant activity. To search for antioxidants in L. aureum, an optimized, on-line high performance liquid chromatography (HPLC) method with DPPH (1,1-diphenyl-2-picrylhydrazyl) for the detection of radical scavenging ability was developed. Response surface methodology (RSM) was used to determine the best combination of experimental conditions, such as DPPH concentration, DPPH velocity and the reaction tank length. The optimized method uses a DPPH concentration of 25.0 mu g/mL, DPPH velocity of 0.45 mL/min and a 15.0 m long reaction tank. The highly sensitive, optimized method can not only be used for the detection of antioxidants in plants, but can also be coupled with mass spectrometry (MS) to obtain the mass-to-charge ratios of chemical species corresponding to the different peaks in the HPLC profiles. Seven antioxidants were identified in L. aureum using the optimized method, including myricetin-3-O-beta-D-(6 ''-O-galloyl)-glucopyranoside, myricetin-3-O-glucoside, myricitrin, eriodictyol-7-O-glucoside, myricetin, eriodictyol and homoeridictyol. (C) 2015 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000352039600064 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/13265]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, North West Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Dandan,Chi, Xiaofeng,Dong, Qi,et al. Antioxidants screening in Limonium aureum by optimized on-line HPLC-DPPH assay[J]. INDUSTRIAL CROPS AND PRODUCTS,2015,67:492-497. |
| APA | Geng, Dandan,Chi, Xiaofeng,Dong, Qi,&Hu, Fengzu.(2015).Antioxidants screening in Limonium aureum by optimized on-line HPLC-DPPH assay.INDUSTRIAL CROPS AND PRODUCTS,67,492-497. |
| MLA | Geng, Dandan,et al."Antioxidants screening in Limonium aureum by optimized on-line HPLC-DPPH assay".INDUSTRIAL CROPS AND PRODUCTS 67(2015):492-497. |
入库方式： OAI收割
来源：西北高原生物研究所
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