Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization
文献类型：期刊论文
| 作者 | Ma, Tao2,3,4; Hu, Na2,3; Zhang, Qiulong2,3,4; Ding, Chenxi1; Che, Guodong2,3; Wu, Hongliang2,3; Sun, Yanan2,3,4; Ji, Zhongyin2,3,4; You, Jinmao2,3; Suo, Yourui2,3 |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2018 |
| 卷号 | 41期号:2页码:49-57 |
| 关键词 | Corydalis Microwave Assisted Extraction Pre-column Derivatization Response Surface Methodology Rp-hplc-fld-ms Triterpenic Acids |
| DOI | 10.1080/10826076.2015.1116092 |
| 英文摘要 | A rapid and sensitive reversed-phase high performance liquid chromatography- fluorescence-mass spectrometer (RP-HPLC-FLD-MS) method based on pre-column derivatization using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as labelling reagent has been developed for simultaneous determination of five triterpenic acids (asiatic acid (AA), maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA), and betulinic acid (BA). The presented method was validated for linearity (correlation coefficient R-2>0.9994), precision (RSD 2.1%-3.9%) and reproducibility (RSD 0.01%-2.1%). The limits of detection (LODs) and the limits of quantitation LOQs were within the range of 0.71-1.02 ng mL(-1) and 2.28-3.24 ng mL(-1), respectively. The proposed method was successfully applied to simultaneously determine five triterpenic acids of four Corydalis plants and showed satisfactory reproducibility and credibility. Moreover, several main parameters affecting extraction procedure and derivatization efficiency were investigated by response surface methodology (RSM), respectively. Triterpenic acid content in four Corydalis plants was measured according to the established method and the results indicated that triterpenic acid contents were various in different organs and herbs. |
| 语种 | 英语 |
| WOS记录号 | WOS:000424808500001 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/13333]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qinghai Red Cross Hosp, Xining, Qinghai, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, 23 Xinning Rd, Xining 810001, Qinghai, Peoples R China 3.Qinghai Prov Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Tao,Hu, Na,Zhang, Qiulong,et al. Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2018,41(2):49-57. |
| APA | Ma, Tao.,Hu, Na.,Zhang, Qiulong.,Ding, Chenxi.,Che, Guodong.,...&Ding, Chenxu.(2018).Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,41(2),49-57. |
| MLA | Ma, Tao,et al."Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 41.2(2018):49-57. |
入库方式： OAI收割
来源：西北高原生物研究所
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