Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC
文献类型：期刊论文
| 作者 | Lei, Tianxiang2,5; Wang, Huan3; Li, Songling1; Shen, Jianwei3; Chen, Shilong2,4; Cai, Xiaojian1; Zhou, Dangwei2,4,5 |
| 刊名 | 3 BIOTECH
 |
| 出版日期 | 2018-03-13 |
| 卷号 | 8 |
| 关键词 | Przewalskia Tangutica Maxim Hairy Root Tropane Alkaloid Hplc |
| DOI | 10.1007/s13205-018-1203-5 |
| 英文摘要 | To achieve a high yield of tropane alkaloids (TA) and exploit the alpine plant sustainably, an optimized protocol for induction and establishment of hairy roots culture of Prezwalskia tangutica Maxim was developed through selection of appropriate Agrobacterium strain and the explant type. The hypocotyl is more readily facile to induce the HR than the cotyledon is when infected with the three different agrobacterium strains. MUS440 has an efficiency (of up to 20%), whereas the ATCC10060 (A4) can induce HR on both types of explants with the highest frequency (33.33%), root length (21.17 +/- 2.84 cm), and root number (10.83 +/- 1.43) per explant than the other strains. The highest HR production resulted from using hypocotyl as explants. Independent transformed HR was able to grow vigorously and to propagate on a no-hormone 1/2MS liquid medium. The presence of pRi rolB gene in transformation of HR was confirmed by PCR amplification. In the liquid medium, the HR growth curve appeared to be "S" shaped, and ADB had increased to 4.633 g/l. Moreover, HPLC analysis showed that HR lines have an extraordinary ability to produce atropine (229.88 mg/100 g), anisodine (4.09 mg/100 g), anisodamine (12.85 mg/100 g), and scopolamine (10.69 mg/100 g), which were all more significant than the control roots. In conclusion, our study optimized the culture condition and established a feasible genetics reactor for P. tangutica green exploration and biological study in the alpine region. |
| 语种 | 英语 |
| WOS记录号 | WOS:000427628700001 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/13338]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qinghai Univ, Inst Soil & Fertilizer, Xining 810008, Qinghai, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810008, Qinghai, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Tibetan Med Ctr, Xining 810008, Qinghai, Peoples R China 4.Chinese Acad Sci, Key Lab Qinghai Plant Mol Breeding, Xining 810008, Qinghai, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Tianxiang,Wang, Huan,Li, Songling,et al. Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC[J]. 3 BIOTECH,2018,8. |
| APA | Lei, Tianxiang.,Wang, Huan.,Li, Songling.,Shen, Jianwei.,Chen, Shilong.,...&Zhou, Dangwei.(2018).Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC.3 BIOTECH,8. |
| MLA | Lei, Tianxiang,et al."Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC".3 BIOTECH 8(2018). |
入库方式： OAI收割
来源：西北高原生物研究所
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