Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation
文献类型：期刊论文
| 作者 | Zhang, Sufang2,3; Zhao, Zongbao Kent2,3; Liu, Hongdi1,2; Jiao, Xiang2; Wang, Yanan2; Yang, Xiaobing2,3; Sun, Wenyi2; Wang, Jihui1 |
| 刊名 | FEMS YEAST RESEARCH
 |
| 出版日期 | 2017-03-01 |
| 卷号 | 17期号:2 |
| 关键词 | Electroporation Linear Dna Fragment Rhodosporidium Toruloides Genetic Transformation Oleaginous Yeast |
| DOI | 10.1093/femsyr/fox017 |
| 英文摘要 | Metabolic engineering of Rhodosporidium toruloides, a robust lipid and caroteinoid producer, is of great importance for oleochemicals and carotenoids production. However, the Agrobacterium-mediated gene transformation is tedious and time consuming. Here, we described a fast and efficient genetic transformation of R. toruloides using electroporation with linear DNA fragments, and the process was optimized. The results showed that 2 x 10(3) transformants can be obtained at 0.7 kV/mu g linear DNA by using hygromycin and bleomycin as selection markers after the competent cells pretreated with 25 mM DTT and 100 mM LiAc. Our results would facilitate mutant library construction and metabolic engineering of R. toruloides for production of oleochemicals and carotenoids. We further demonstrated that all transformants arose due to illegitimate integration of transforming DNA fragments by colony PCR. |
| 语种 | 英语 |
| WOS记录号 | WOS:000400577600007 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/152011]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Sufang,Zhao, Zongbao Kent,Liu, Hongdi,et al. Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation[J]. FEMS YEAST RESEARCH,2017,17(2). |
| APA | Zhang, Sufang.,Zhao, Zongbao Kent.,Liu, Hongdi.,Jiao, Xiang.,Wang, Yanan.,...&Wang, Jihui.(2017).Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation.FEMS YEAST RESEARCH,17(2). |
| MLA | Zhang, Sufang,et al."Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation".FEMS YEAST RESEARCH 17.2(2017). |
入库方式： OAI收割
来源：大连化学物理研究所
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