The Null-Test for peptide identification algorithm in Shotgun proteomics
文献类型：期刊论文
| 作者 | Zhou, Yuan2; Zhang, Yu-Kui2; Zhang, Shu-Rong2; Shan, Yi-Chu2; Jiang, Hao1,2; Liu, Jian-Hui1,2; Zhang, Li-Hua2 |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2017-06-23 |
| 卷号 | 163页码:118-125 |
| 关键词 | Null-test Shotgun Proteomics Peptide Identification Target-decoy Search Fdr Estimation |
| DOI | 10.1016/j.jprot.2017.05.010 |
| 英文摘要 | The present research proposed general evaluation strategy named Null-Test for peptide identification algorithm in Shotgun proteomics. The Null-Test method based on random matching can be utilized to check whether the algorithm has a tendency to make a mistake or has potential bugs, faultiness, errors etc., and to validate the reliability of the identification algorithm. Unfortunately, none of the five famous identification software could pass the most stringent Null-Test. PatternLab had good performance in both Null-Test and routine search by making a good control on the overfitting with sound design. The fuzzy logics based method presented as another candidate strategy could pass the Null-Test and has competitive efficiency in peptide identification. Filtering the results by appropriate FDR would increase the number of discoveries in an experiment, at the cost of losing control of Type I errors. Thus, it is necessary to utilize some more stringent criteria when someone wants to design or analyze an algorithm/software. The more stringent criteria will facilitate the discovery of latent bugs, faultiness, errors etc. in the algorithm/software. It would be recommended to utilize independent search combining random database with statistics theorem to estimate the accurate FDR of the identified results. |
| 语种 | 英语 |
| WOS记录号 | WOS:000404709600010 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/152085]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yuan,Zhang, Yu-Kui,Zhang, Shu-Rong,et al. The Null-Test for peptide identification algorithm in Shotgun proteomics[J]. JOURNAL OF PROTEOMICS,2017,163:118-125. |
| APA | Zhou, Yuan.,Zhang, Yu-Kui.,Zhang, Shu-Rong.,Shan, Yi-Chu.,Jiang, Hao.,...&Zhang, Li-Hua.(2017).The Null-Test for peptide identification algorithm in Shotgun proteomics.JOURNAL OF PROTEOMICS,163,118-125. |
| MLA | Zhou, Yuan,et al."The Null-Test for peptide identification algorithm in Shotgun proteomics".JOURNAL OF PROTEOMICS 163(2017):118-125. |
入库方式： OAI收割
来源：大连化学物理研究所
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