Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry
文献类型：期刊论文
| 作者 | Li, Wenxue2; Petruzziello, Filomena2; Zhao, Nan1; Zhao, Huiyuan1; Ye, Xueting3; Zhang, Xiaozhe1; Rainer, Gregor2 |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2017-06-17 |
| 卷号 | 17 |
| 关键词 | High-resolution Mass Spectrometry Microproteins Mouse Brain Peptides Top Down |
| DOI | 10.1002/pmic.201600419 |
| 英文摘要 | Microproteins and endogenous peptides in the brain contain important substances that have critical roles in diverse biological processes, contributing to signal transduction and intercellular signaling. However, variability in their physical or chemical characteristics, such as molecule size, hydrophobicity, and charge states, complicate the simultaneous analysis of these compounds, although this would be highly beneficial for the field of neuroscience research. Here, we present a top-down analytical method for simultaneous analysis of microproteins and endogenous peptides using high-resolution nanocapillary LC-MS/MS. This method is detergent-free and digestion-free, which allows for extracting and preserving intact microproteins and peptides for direct LC-MS analysis. Both higher energy collision dissociation and electron-transfer dissociation fragmentations were used in the LC-MS analysis to increase the identification rate, and bioinformatics tools ProteinGoggle and PEAKS Studio software were utilized for database search. In total, we identified 471 microproteins containing 736 proteoforms, including brain-derived neurotrophic factor and a number of fibroblast growth factors. In addition, we identified 599 peptides containing 151 known or potential neuropeptides such as somatostatin-28 and neuropeptide Y. Our approach bridges the gap for the characterization of brain microproteins and peptides, which permits quantification of a diversity of signaling molecules for biomarker discovery or therapy diagnosis in the future. |
| 语种 | 英语 |
| WOS记录号 | WOS:000403899900006 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/152112]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Fribourg, Visual Cognit Lab, Dept Med, Fribourg, Switzerland 3.Shenyang Pharmaceut Univ, Shenyang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wenxue,Petruzziello, Filomena,Zhao, Nan,et al. Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry[J]. PROTEOMICS,2017,17. |
| APA | Li, Wenxue.,Petruzziello, Filomena.,Zhao, Nan.,Zhao, Huiyuan.,Ye, Xueting.,...&Rainer, Gregor.(2017).Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry.PROTEOMICS,17. |
| MLA | Li, Wenxue,et al."Separation and identification of mouse brain tissue microproteins using top-down method with high resolution nanocapillary liquid chromatography mass spectrometry".PROTEOMICS 17(2017). |
入库方式： OAI收割
来源：大连化学物理研究所
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