Ultrasensitive Detection of Hair Cortisol Based on Portable Raman Spectrometer and Double-layer Paper Microdevice
文献类型：期刊论文
| 作者 | Gao Zhigang2; Zheng Tingting2; Deng Jiu2; Li Xiaorui2; Qu Yueyang2; Lu Yao3; Liu Tingjiao1; Luo Yong2; Zhao Weijie2; Lin Bingcheng2 |
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2017-04-15 |
| 卷号 | 75页码:355-359 |
| 关键词 | Hair Cortisol Sers Paper Microdevice Portable Raman Spectrometer Competitive Immunoassay |
| DOI | 10.6023/A16110598 |
| 英文摘要 | Cortisol in the human hair is a clinical biomarker of long-term social-and-work-related mental stress, which is of high morbidity rate in the current modern society. This study developed a sensitive hair cortisol assay, featuring surface- enhanced Raman spectroscopy, immunoreaction, and a double-layer paper microdevice. The first layer of the paper microdevice was used to remove the hair residue in the hair extract by filtration (sample pretreatment). The second layer was used for competitive immunoreaction and detection. Standard cortisol antigen immobilized in the second layer and the free cortisol in hair extract competed to bind the spiked Raman-active cortisol monoclonal antibody solution. The hair cortisol can be quantitated by the intensity of Raman signal of monoclonal antibody bound on the paper. We found the Raman signal decreased as the cortisol concentration increases in hair samples. The relative Raman intensity measured was linearly proportional to the logarithmic value of the cortisol concentration in hair samples we measured. The detection of limit (LOD) was 1 pg/mL with the portable Raman spectrometer. The RSD of measurement was 8.38% (n=6). In addition, we used LC-MS to measure two real samples as a comparison with our method as above. The results are 0.771 and 0.153 ng/mL by LC-MS method and 0.63 and 0.247 ng/mL by the proposed method. It can be observed that the results are in same order, demonstrating the validity of the proposed method. In addition, 48 samples can be measured in a single chip. These results showed that this method is sensitive, specific, and suitable for large-scale screening of hair cortisol samples. |
| 语种 | 英语 |
| WOS记录号 | WOS:000402486800003 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/152197]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Med Univ, Coll Stomatol, Dalian 116044, Peoples R China 2.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116024, Peoples R China 3.Dalian Inst Chem Phys, Biol Div, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao Zhigang,Zheng Tingting,Deng Jiu,et al. Ultrasensitive Detection of Hair Cortisol Based on Portable Raman Spectrometer and Double-layer Paper Microdevice[J]. ACTA CHIMICA SINICA,2017,75:355-359. |
| APA | Gao Zhigang.,Zheng Tingting.,Deng Jiu.,Li Xiaorui.,Qu Yueyang.,...&Lin Bingcheng.(2017).Ultrasensitive Detection of Hair Cortisol Based on Portable Raman Spectrometer and Double-layer Paper Microdevice.ACTA CHIMICA SINICA,75,355-359. |
| MLA | Gao Zhigang,et al."Ultrasensitive Detection of Hair Cortisol Based on Portable Raman Spectrometer and Double-layer Paper Microdevice".ACTA CHIMICA SINICA 75(2017):355-359. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。