Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides
文献类型：期刊论文
| 作者 | Sun, Xudong2; Dong, Jing1; Li, Jinan1; Ye, Mingliang1; Zhang, Weibing2; Ou, Junjie1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2017-05-19 |
| 卷号 | 1498页码:72-79 |
| 关键词 | Glycopeptides Macroporous Adsorption Resin Layer-by-layer Hydrophilic Interaction Liquid Chromatography Solid-phase Extraction |
| DOI | 10.1016/j.chroma.2016.12.045 |
| 英文摘要 | A facile approach for preparation of hydrophilic polysaccharide-functionalized macroporous adsorption resin (MAR) was presented. Polydisperse MAR with approximately 250 mu m in diameter was synthesized via suspension polymerization and then modified with polysaccharides, sodium hyaluronate (HA) and chitosan (CS), through layer-by-layer (LbL) self-assembly pro cess. The preparation method was facile, low cost and easy to expand production from tens of grams to one kilogram. The resulting MAR@(HA/CS)(20) possesses highly hydrophilic property and rapid adsorption behavior. The specificity and efficiency of MAR@(HA/CS)(20) to glycopeptide was demonstrated by trapping N-linked glycopeptide from tryptic digests of human immunoglobulin G (IgG) and horseradish peroxidase (HRP) with a homemade solid-phase extraction (SPE) column. The detection sensitivity for glycopeptide determined by MALDI-TOF MS was as low as 5 fmol for standard tryptic digest of human IgG. The enrichment recoveries were higher than 73%. Furthermore, in the analysis of real biological sample, 745 unique N-glycosylation sites in 1353 unique glycopeptides mapped to 379 N-glycosylated proteins were identified in three replicate analyses of protein sample extracted from mouse liver, showing the great potential of MAR@(HA/CS)(20) in the enrichment and identification of low-abundance N-linked glycopeptides in complicated biological samples. (C) 2016 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000401043000010 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/152275]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Xudong,Dong, Jing,Li, Jinan,et al. Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:72-79. |
| APA | Sun, Xudong,Dong, Jing,Li, Jinan,Ye, Mingliang,Zhang, Weibing,&Ou, Junjie.(2017).Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides.JOURNAL OF CHROMATOGRAPHY A,1498,72-79. |
| MLA | Sun, Xudong,et al."Facile preparation of polysaccharide functionalized macroporous adsorption resin for highly selective enrichment of glycopeptides".JOURNAL OF CHROMATOGRAPHY A 1498(2017):72-79. |
入库方式： OAI收割
来源：大连化学物理研究所
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