Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals
文献类型：期刊论文
| 作者 | Yu, Tao2,3; Zhou, Yongjin J.2,3,6; Wenning, Leonie2,3; Liu, Quanli2,3; Krivoruchko, Anastasia2,3,4; Siewers, Verena2,3; Nielsen, Jens1,2,3,5; David, Florian2,3,4 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2017-05-26 |
| 卷号 | 8 |
| DOI | 10.1038/ncomms15587 |
| 英文摘要 | Production of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S. cerevisiae. VLCFAs can be selectively modified towards the fatty alcohol docosanol (C22H46O) by expressing a specific fatty acid reductase. Expression of this enzyme is shown to impair cell growth due to consumption of VLCFA-CoAs. We therefore implement a dynamic control strategy for separating cell growth from docosanol production. We successfully establish high-level and selective docosanol production of 83.5 mg l(-1) in yeast. This approach will provide a universal strategy towards the production of similar high value chemicals in a more scalable, stable and sustainable manner. |
| 语种 | 英语 |
| WOS记录号 | WOS:000402047300001 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/152292]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Royal Inst Technol, Sci Life Lab, SE-17121 Stockholm, Sweden 2.Chalmers, Dept Biol & Biol Engn, Kemivagen 10, SE-41296 Gothenburg, Sweden 3.Chalmers, Novo Nordisk Fdn, Ctr Biosustainabil, SE-41296 Gothenburg, Sweden 4.Chalmers, Biopetrolia AB, Syst & Synthet Biol Grp, Kemivagen 10, SE-41296 Gothenburg, Sweden 5.Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, DK-2800 Lyngby, Denmark 6.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Tao,Zhou, Yongjin J.,Wenning, Leonie,et al. Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals[J]. NATURE COMMUNICATIONS,2017,8. |
| APA | Yu, Tao.,Zhou, Yongjin J..,Wenning, Leonie.,Liu, Quanli.,Krivoruchko, Anastasia.,...&David, Florian.(2017).Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals.NATURE COMMUNICATIONS,8. |
| MLA | Yu, Tao,et al."Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals".NATURE COMMUNICATIONS 8(2017). |
入库方式： OAI收割
来源：大连化学物理研究所
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