Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography
文献类型：期刊论文
| 作者 | Liu, Shengju2,3; Peng, Jiaxi2,3; Zhang, Hongyan2,3; Li, Xin2,3; Liu, Zheyi2,3; Kang, Xiaohui2; Wu, Minghuo1; Wu, Ren'an2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2017-05-19 |
| 卷号 | 1498页码:64-71 |
| 关键词 | Mesoporous Carbon Nanoparticles Organic-silica Hybrid Monolithic Column Capillary Liquid Chromatography (Clc) Small Molecule Protein Peptide |
| DOI | 10.1016/j.chroma.2017.03.067 |
| 英文摘要 | An organic-silica hybrid monolithic capillary column was fabricated by crosslinking (3-aminopropyl)trimethoxysilane (APTMS) modified mesoporous carbon nanoparticles (AP-MCNs) with tetramethoxysilane (TMOS) and n-butyltrimethoxysilane (C4-TriMOS). Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy, mercury intrusion porosimetry and inverse size-exclusion chromatography characterization proved the successful immobilization of mesoporous carbon nanoparticles (MCNs). The crosslinking of AP-MCNs into the hybrid monolithic matrix has significantly increased the reversed-phase retention of alkylbenzenes and chromatographic performance for small molecules separations in comparison with the neat one without MCNs. The resulting column efficiency of the mesoporous carbon nanoparticle-based butyl-silica hybrid monolithic column (MCN-C4-monolith) was up to ca. 116,600 N/m for the capillary liquid chromatography (cLC) separation of butylbenzene. Enhanced performance of proteins separation was achieved on the MCN-C4-monolith in comparison with the butyl-silica hybrid monolithic column without MCN (C4-monolith). The separation of peptides from bovine serum albumin (BSA) digest was carried out on the MCN-C4-monolith by capillary liquid chromatography-tandem mass spectrometry (cLC-MS/MS) with protein sequence coverage of 81.9%, suggesting its potential application in proteomics. (C) 2017 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000401043000009 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/152294]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ Technol, Sch Food & Environm, Panjin 124221, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Shengju,Peng, Jiaxi,Zhang, Hongyan,et al. Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:64-71. |
| APA | Liu, Shengju.,Peng, Jiaxi.,Zhang, Hongyan.,Li, Xin.,Liu, Zheyi.,...&Wu, Ren'an.(2017).Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1498,64-71. |
| MLA | Liu, Shengju,et al."Preparation of organic-silica hybrid monolithic columns via crosslinking of functionalized mesoporous carbon nanoparticles for capillary liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1498(2017):64-71. |
入库方式： OAI收割
来源：大连化学物理研究所
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