中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma

文献类型:期刊论文

作者Ke, Zhigang1; Huang, Qing1,2
刊名PLASMA PROCESSES AND POLYMERS
出版日期2013-08-01
卷号10期号:8页码:731-739
关键词Horseradish Peroxidase Hydrogen Peroxide Non-thermal Plasma Optical Spectroscopy Radicals
DOI10.1002/ppap.201300035
文献子类Article
英文摘要The effects of non-thermal plasma at the argon gas-solution interface on horseradish peroxidase (HRP), a classical heme-containing protein, were assessed quantitatively. Evidence from spectroscopic approaches showed that enzymatic activity together with the contents of heme and iron in HRP decreased dramatically and concomitantly upon plasma treatment. The plasma induced both long- and short-lived reactive species which inactivated HRP in the solution. In particular, hydrogen peroxide broke the heme prosthetic group into fluorescent products, mainly dipyrrols and their derivatives, while other factors such as hydroxyl radicals and UV radiation generated during radicals and UV radiation generated during plasma discharge accelerated this heme protein degradation process.
WOS关键词DIELECTRIC BARRIER DISCHARGE ; GAS-SOLUTION INTERFACE ; ATMOSPHERIC-PRESSURE PLASMA ; M-CHLOROPEROXYBENZOIC ACID ; HYDROGEN-PEROXIDE ; INDUCED RADIOLYSIS ; NONTHERMAL PLASMA ; AQUEOUS-SOLUTION ; OXYGEN ; WATER
WOS研究方向Physics ; Polymer Science
语种英语
WOS记录号WOS:000327790000007
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22198]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714
Ke, Zhigang,Huang, Qing. Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma[J]. PLASMA PROCESSES AND POLYMERS,2013,10(8):731-739.
APA Ke, Zhigang,&Huang, Qing.(2013).Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma.PLASMA PROCESSES AND POLYMERS,10(8),731-739.
MLA Ke, Zhigang,et al."Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma".PLASMA PROCESSES AND POLYMERS 10.8(2013):731-739.

入库方式: OAI收割

来源:合肥物质科学研究院

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