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  2. 合肥物质科学研究院
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  4. 技术生物与农业工程研究所
Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb.

文献类型：期刊论文

作者Hou, Jinyan1; Wu, Ying1,2,3; Shen, Yangcheng1; Mao, Yingji1,2; Liu, Wenbo1,2; Zhao, Weiwei1; Mu, Yan1; Li, Minghao1; Yang, Minglei1; Wu, Lifang1,2
刊名SCIENTIA HORTICULTURAE
出版日期2015-12-14
卷号197期号:无页码:218-225
关键词Chinese Tallow Tree Somatic Embryogenesis Mechanical Damage Secondary Somatic Embryos Adventitious Shoots Plant Regeneration
DOI10.1016/j.scienta.2015.09.040
文献子类Article
英文摘要Sapium sebiferium Roxb. is a highly valued ornamental plant species in tropical and subtropical regions. Here we present an efficient protocol for plant regeneration from immature zygotic embryos (IZEs) of S. sebiferium through direct somatic embryogenesis and shoot organogenesis for the first time. The results showed that mechanical damage, plant growth regulators (PGRs) and light regimes had great influence on in vitro morphogenesis. High frequency of somatic embryogenesis was obtained when the mechanical damaged IZEs were cultured on the Murashige and Skoog (MS) medium containing 1.0 mgl(-1) indole-3-acetic acid (IAA) under continuous darkness for two weeks followed by a light/dark photoperiod cycle for four weeks. The induction frequency of somatic embryogenesis was up to 90.0%. Somatic embryos (SEs) at early cotyledonary stage showed highest proliferation frequency (81.7%) and 80.0% of secondary somatic embryos (SSEs) germinated on plant growth regulators (PGRs)-free MS medium. For direct shoot induction pathway, the best shoot regeneration rate (63.3%) was obtained on the medium supplemented with 1.0 mgl-1 6-benzyladenine (6-BA). The plantlets derived from two ways were successfully acclimatized in the greenhouse with more than 90% survival rate. This study will be beneficial for the rescue of immature embryo, inheritance of heterosis as well as the genetic improvement of S. sebiferium. (C) 2015 Elsevier B.V. All rights reserved.
WOS关键词CHINESE TALLOW TREE ; MEDICINAL-PLANT ; TISSUE-CULTURE ; L. ; GROWTH ; BALDCYPRESS ; INITIATION ; INDUCTION ; HYPOCOTYL ; LEAF
WOS研究方向Agriculture
语种英语
WOS记录号WOS:000367411400028
资助机构Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; National Nature Science Foundation of China(11375232) ; National Nature Science Foundation of China(11375232) ; National Nature Science Foundation of China(11375232) ; National Nature Science Foundation of China(11375232) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; Special Fund for Forest Scientific Research in the Public Welfare(201404110) ; National Nature Science Foundation of China(11375232) ; National Nature Science Foundation of China(11375232) ; National Nature Science Foundation of China(11375232) ; National Nature Science Foundation of China(11375232) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Chinese Academy of Sciences(KFJ-EW-STS-083) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Anhui Provincial Natural science Foundation(1408085QC64) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006) ; Fund of Hefei Center of Physical Science and Technology(2012FXCX006)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22210]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn & Bioenergy, Forest Res Ctr,State Forestry Adm, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
3.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471023, Henan, Peoples R China
推荐引用方式
GB/T 7714		 Hou, Jinyan,Wu, Ying,Shen, Yangcheng,et al. Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb.[J]. SCIENTIA HORTICULTURAE,2015,197(无):218-225.
APA Hou, Jinyan.,Wu, Ying.,Shen, Yangcheng.,Mao, Yingji.,Liu, Wenbo.,...&Wu, Lifang.(2015).Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb..SCIENTIA HORTICULTURAE,197(无),218-225.
MLA Hou, Jinyan,et al."Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb.".SCIENTIA HORTICULTURAE 197.无(2015):218-225.

入库方式： OAI收割

来源：合肥物质科学研究院

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