Enhancing the performance of a phospholipase A(1) for oil degumming by bio-imprinting and immobilization
文献类型：期刊论文
| 作者 | Li, Zhemin; Liu, Hui; Zhao, Genhai ; Wang, Peng; Wang, Li; Wu, Hefang; Fang, Xue; Sun, Xiaowen; Wu, Xihua; Zheng, Zhiming
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| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
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| 出版日期 | 2016 |
| 卷号 | 123期号:无页码:122-131 |
| 关键词 | Bio-imprinting Immobilization Phospholipase Miscella Enzymatic Degumming |
| DOI | 10.1016/j.molcatb.2015.11.018 |
| 文献子类 | Article |
| 英文摘要 | When phospholipase A(1) (PLA(1)) was bio-imprinted with 5% soybean lecithin at pH 5.0, its phospholipase (PLA) activity in lecithin-hexane solution increased by 30.9-fold and its substrate specificity was substantially enhanced. A further increase in PLA activity of the bio-imprinted PLA(1) (bi-PLA(1)) from 562 U/g to 1288 U/g was obtained when 105 mg diatomite/mg bi-PLA(1) was incorporated as an immobilization carrier. The degumming abilities of PLA(1), bi-PLA(1), and immobilized bi-PLA(1)) (im-bi-PLA(1)) in crude soybean oil and crude oil-hexane (1:1 w/w) miscella were also investigated. The phosphorus content in the crude soybean oil was reduced to 7.3 mg/kg from an initial level of 406.9 mg/kg after incubation with 100 mg/kg im-bi-PLA(1) and 2% water at 50 degrees C for 180 min. In miscella degumming, the residual phosphorus content was reduced from 406.9 mg/kg to 18.7 mg/kg by incubation with 100 mg/kg im-bi-PLA(1) and 2% water at 50 degrees C for 180 min and neutralization with 3 M NaOH solution. In comparison, the original PLA(1) reduced the residual phosphorus content to 62.9 mg/kg and 92.4 mg/kg in crude oil and miscella degumming, respectively. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | CANDIDA-RUGOSA LIPASE ; ORGANIC-SOLVENTS ; VEGETABLE-OILS ; SOYBEAN OIL ; HYDROLYSIS ; MEDIA ; TRANSESTERIFICATION ; BIOCATALYST ; ACTIVATION ; BIOSENSOR |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000369681800018 |
| 资助机构 | Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Key 863 Fund of China(2014AA021704) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) ; Anhui Provincial Natural Science Foundation(1308085MA07) |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/22309]  |
| 专题 | 合肥物质科学研究院_技术生物与农业工程研究所 |
| 作者单位 | Chinese Acad Sci, Inst Biotechnol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Zhemin,Liu, Hui,Zhao, Genhai,et al. Enhancing the performance of a phospholipase A(1) for oil degumming by bio-imprinting and immobilization[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2016,123(无):122-131. |
| APA | Li, Zhemin.,Liu, Hui.,Zhao, Genhai.,Wang, Peng.,Wang, Li.,...&Zheng, Zhiming.(2016).Enhancing the performance of a phospholipase A(1) for oil degumming by bio-imprinting and immobilization.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,123(无),122-131. |
| MLA | Li, Zhemin,et al."Enhancing the performance of a phospholipase A(1) for oil degumming by bio-imprinting and immobilization".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 123.无(2016):122-131. |
入库方式： OAI收割
来源：合肥物质科学研究院
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