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Highly Sensitive and Selective Surface-Enhanced Raman Spectroscopy Label-free Detection of 3,3 ',4,4 '-Tetrachlorobiphenyl Using DNA Aptamer-Modified Ag-Nanorod Arrays

文献类型：期刊论文

作者Sun, Kexi1; Huang, Qing1,4; Meng, Guowen2,3,4; Lu, Yilin1
刊名ACS APPLIED MATERIALS & INTERFACES
出版日期2016-03-02
卷号8期号:8页码:5723-5728
关键词Surface-enhanced Raman Spectroscopy (Sers) Label-free Detection Aptamers Polychlorinated Biphenyls Ag-nanorod Arrays
DOI10.1021/acsami.5b12866
文献子类Article
英文摘要An Unproved label-free approach for highly sensitive and selective detection of 3,3',4,4'-teirachlorobiphenyl (PCB-77), a type of polychlorinated biphenyl, via surface-enhanced Raman spectroscopy (SERS) using DNA aptamer-modified Ag-nanorod arrays as the effective substrate is reported. The devised system consists of Ag-nanorod (Ag-NR) arrays with the PCB-77 binding aptamers anchored covalently to the Ag surfaces through a thiol linker: The aptamers are made of single-stranded DNA (ssDNA) oligomers, with one end standing on the Ag surface, and upon conjugation with PCB-77, the ssDNA molecules can change their conformation to hairpin loops, so that the Raman intensity of guanines at the other end of the DNA Strand increases accordingly. As such, the intensity ratio I(656 cm(-1))/I(733 Cm-1) increases concomitantly with the increase of the concentration of PCB-77, making the quantitative evaluation of trace amounts of PCB-77 attainable. Moreover, it is found that the DNA aptamer-based Ag-NR arrays can be more responsive with a lower and, optimal density of the DNA molecules Modified on the substrate surface, and the best sensitivity for detection of PCB-77 can be achieved with with the lower detection limit approaching 33 X 10(-8) M. This work therefore demonstrates that the design, of aptamer-modified Ag-NRS can be used as practically promising SERS substrate for label-free trace detection of persistent organic pollutants (FOPS) in the environment.
WOS关键词POLYCHLORINATED-BIPHENYLS ; SCATTERING ; NANOPARTICLES ; CONGENERS
WOS研究方向Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:000371453600078
资助机构National Basic Research Program of China(2013CB934304) ; National Basic Research Program of China(2013CB934304) ; National Basic Research Program of China(2013CB934304) ; National Basic Research Program of China(2013CB934304) ; National Natural Science Foundation of China(11175204) ; National Natural Science Foundation of China(11175204) ; National Natural Science Foundation of China(11175204) ; National Natural Science Foundation of China(11175204) ; Cross-disciplinary Collaborative Teams Program of CAS ; Cross-disciplinary Collaborative Teams Program of CAS ; Cross-disciplinary Collaborative Teams Program of CAS ; Cross-disciplinary Collaborative Teams Program of CAS ; Natural Science Foundation of Anhui Province, China(Y49AH51586) ; Natural Science Foundation of Anhui Province, China(Y49AH51586) ; Natural Science Foundation of Anhui Province, China(Y49AH51586) ; Natural Science Foundation of Anhui Province, China(Y49AH51586) ; National Basic Research Program of China(2013CB934304) ; National Basic Research Program of China(2013CB934304) ; National Basic Research Program of China(2013CB934304) ; National Basic Research Program of China(2013CB934304) ; National Natural Science Foundation of China(11175204) ; National Natural Science Foundation of China(11175204) ; National Natural Science Foundation of China(11175204) ; National Natural Science Foundation of China(11175204) ; Cross-disciplinary Collaborative Teams Program of CAS ; Cross-disciplinary Collaborative Teams Program of CAS ; Cross-disciplinary Collaborative Teams Program of CAS ; Cross-disciplinary Collaborative Teams Program of CAS ; Natural Science Foundation of Anhui Province, China(Y49AH51586) ; Natural Science Foundation of Anhui Province, China(Y49AH51586) ; Natural Science Foundation of Anhui Province, China(Y49AH51586) ; Natural Science Foundation of Anhui Province, China(Y49AH51586)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22324]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn,Key Lab Ion Beam Bioeng, Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Anhui, Peoples R China
2.Chinese Acad Sci, Inst Solid State Phys, Key Lab Mat Phys, Hefei 230031, Anhui, Peoples R China
3.Chinese Acad Sci, Inst Solid State Phys, Anhui Key Lab Nanomat & Nanotechnol, Hefei 230031, Anhui, Peoples R China
4.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Kexi,Huang, Qing,Meng, Guowen,et al. Highly Sensitive and Selective Surface-Enhanced Raman Spectroscopy Label-free Detection of 3,3 ',4,4 '-Tetrachlorobiphenyl Using DNA Aptamer-Modified Ag-Nanorod Arrays[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(8):5723-5728.
APA Sun, Kexi,Huang, Qing,Meng, Guowen,&Lu, Yilin.(2016).Highly Sensitive and Selective Surface-Enhanced Raman Spectroscopy Label-free Detection of 3,3 ',4,4 '-Tetrachlorobiphenyl Using DNA Aptamer-Modified Ag-Nanorod Arrays.ACS APPLIED MATERIALS & INTERFACES,8(8),5723-5728.
MLA Sun, Kexi,et al."Highly Sensitive and Selective Surface-Enhanced Raman Spectroscopy Label-free Detection of 3,3 ',4,4 '-Tetrachlorobiphenyl Using DNA Aptamer-Modified Ag-Nanorod Arrays".ACS APPLIED MATERIALS & INTERFACES 8.8(2016):5723-5728.

入库方式： OAI收割

来源：合肥物质科学研究院

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