The role of protein kinase C alpha translocation in radiation-induced bystander effect
文献类型：期刊论文
| 作者 | Fang, Zihui1; Xu, An2 ; Wu, Lijun2; Hei, Tom K.3; Hong, Mei1
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-05-11 |
| 卷号 | 6期号:无页码:1-9 |
| DOI | 10.1038/srep25817 |
| 文献子类 | Article |
| 英文摘要 | Ionizing radiation is a well known human carcinogen. Evidence accumulated over the past decade suggested that extranuclear/extracellular targets and events may also play a critical role in modulating biological responses to ionizing radiation. However, the underlying mechanism(s) of radiation-induced bystander effect is still unclear. In the current study, A(L) cells were irradiated with alpha particles and responses of bystander cells were investigated. We found out that in bystander AL cells, protein kinase C alpha (PKCZ alpha) translocated from cytosol to membrane fraction. Pre-treatment of cells with PKC translocation inhibitor chelerythrine chloride suppressed the induced extracellular signal-regulated kinases (ERK) activity and the increased cyclooxygenase 2 (COX-2) expression as well as the mutagenic effect in bystander cells. Furthermore, tumor necrosis factor alpha (TNF alpha) was elevated in directly irradiated but not bystander cells; while TNFa receptor 1 (TNFR1) increased in the membrane fraction of bystander cells. Further analysis revealed that PKC activation caused accelerated internalization and recycling of TNFR1. Our data suggested that PKC alpha translocation may occur as an early event in radiation-induced bystander responses and mediate TNF alpha-induced signaling pathways that lead to the activation of ERK and up-regulation of COX-2. |
| WOS关键词 | MOUSE EPIDERMAL-CELLS ; GENOMIC INSTABILITY ; IONIZING-RADIATION ; HUMAN FIBROBLASTS ; MAMMALIAN-CELLS ; PATHWAY ; SENSITIVITY ; ACTIVATION ; EXPRESSION ; PARTICLES |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000375615100001 |
| 资助机构 | National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; 81373473 ; 81373473 ; 81373473 ; 81373473 ; U1332124) ; U1332124) ; U1332124) ; U1332124) ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; 81373473 ; 81373473 ; 81373473 ; 81373473 ; U1332124) ; U1332124) ; U1332124) ; U1332124) |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/22381]  |
| 专题 | 合肥物质科学研究院_技术生物与农业工程研究所 |
| 作者单位 | 1.South China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei 230031, Peoples R China 3.Columbia Univ Coll Phys & Surg, Ctr Radiol Res, 630 W 168th St, New York, NY 10032 USA |
| 推荐引用方式 GB/T 7714 | Fang, Zihui,Xu, An,Wu, Lijun,et al. The role of protein kinase C alpha translocation in radiation-induced bystander effect[J]. SCIENTIFIC REPORTS,2016,6(无):1-9. |
| APA | Fang, Zihui,Xu, An,Wu, Lijun,Hei, Tom K.,&Hong, Mei.(2016).The role of protein kinase C alpha translocation in radiation-induced bystander effect.SCIENTIFIC REPORTS,6(无),1-9. |
| MLA | Fang, Zihui,et al."The role of protein kinase C alpha translocation in radiation-induced bystander effect".SCIENTIFIC REPORTS 6.无(2016):1-9. |
入库方式： OAI收割
来源：合肥物质科学研究院
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