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Antitumor and radiosensitization effect of C-12(6+) heavy-ion irradiation mediated by radiation-inducible gene therapy

文献类型：期刊论文

作者Liu, Hui1; Jin, Chu-Feng1; Ge, Sheng-Fang2; Wu, Li-Jun3
刊名NUCLEAR SCIENCE AND TECHNIQUES
出版日期2016-02-01
卷号27期号:1页码:1-7
关键词Radio Genetic Therapy C-12 Ion irradiatIon Apoptosis Egr-1 Prompter Tumor Necrosis Factor-related Apoptosis-inducing Ligand
DOI10.1007/s41365-016-0021-x
文献子类Article
英文摘要Radio genetic therapy which combines gene therapy with radiotherapy has shown promising results in cancer treatment. In this study, an oncolytic adenovirus-based gene therapy system regulated by radiation was constructed to improve the cancer curative effect. This gene therapy system incorporated the radiation-inducible early growth response gene (Egr-1) promoter and the anticancer gene tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). To confirm the antitumor effect of Ad-ET combined with C-12(6+) ion irradiation, the survival and apoptosis fraction of tumor cells HT1080 and normal cells MRC-5 in combination treatment were detected by CCK-8 assay and FACS analysis. Then the expression levels of TRAIL gene and protein were tested by real-time PCR and western blotting. The results show that C-12(6+) ion irradiation could induce cell growth inhibition and apoptosis by activating the TRAIL gene expression in tumor cells, while exhibiting no obvious toxicity to the normal lung cell line MRC-5. The results also demonstrate that use of an oncolytic adenovirus-based radiation-inducible gene therapy system together with C-12(6+) ion irradiation could cause synergistic antitumor effect specifically in tumor cells but not in normal cells. The results indicate that the novel radio genetic therapy could potentiate radiation treatment by improving the safety and efficiency of monotherapy, and provide theoretical support for clinical application of combination treatment.
WOS关键词PROBABILISTIC SAFETY ASSESSMENT ; IONIZING-RADIATION ; ACCURATE RADIOTHERAPY ; GLIOMA-CELLS ; LIGAND ; CANCER ; APOPTOSIS ; SYSTEM ; TRAIL ; DEATH
WOS研究方向Nuclear Science & Technology ; Physics
语种英语
WOS记录号WOS:000376332800010
资助机构National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Magnetic Confinement Fusion Science Program of China(2014GB112006) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; National Natural Science Foundation of China(11305204) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220) ; Natural Science Foundation of Anhui Province of China(1508085SME220)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22415]  
专题合肥物质科学研究院_中国科学院核能安全技术研究所
作者单位1.Chinese Acad Sci, Inst Nucl Energy Safety Technol, Key Lab Neutron & Radiat Safety, Hefei 230031, Peoples R China
2.Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Shanghai 200025, Peoples R China
3.Chinese Acad Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Hui,Jin, Chu-Feng,Ge, Sheng-Fang,et al. Antitumor and radiosensitization effect of C-12(6+) heavy-ion irradiation mediated by radiation-inducible gene therapy[J]. NUCLEAR SCIENCE AND TECHNIQUES,2016,27(1):1-7.
APA Liu, Hui,Jin, Chu-Feng,Ge, Sheng-Fang,&Wu, Li-Jun.(2016).Antitumor and radiosensitization effect of C-12(6+) heavy-ion irradiation mediated by radiation-inducible gene therapy.NUCLEAR SCIENCE AND TECHNIQUES,27(1),1-7.
MLA Liu, Hui,et al."Antitumor and radiosensitization effect of C-12(6+) heavy-ion irradiation mediated by radiation-inducible gene therapy".NUCLEAR SCIENCE AND TECHNIQUES 27.1(2016):1-7.

入库方式： OAI收割

来源：合肥物质科学研究院

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