Radioadaptive Response for Reproductive Cell Death Demonstrated in In Vivo Tissue Model of Caenorhabditis elegans
文献类型：期刊论文
| 作者 | Tang, Huangqi; Chen, Liangwen; Liu, Jialu; Shi, Jue; Li, Qingqing; Wang, Ting ; Wu, Lijun; Zhan, Furu; Bian, Po
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| 刊名 | RADIATION RESEARCH
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| 出版日期 | 2016-04-01 |
| 卷号 | 185期号:4页码:402-410 |
| DOI | 10.1667/RR14368.1 |
| 文献子类 | Article |
| 英文摘要 | Reproductive cell death (RCD) occurs after one or more cell divisions resulting from an insult such as radiation exposure or other treatments with carcinogens or mutagens. The radioadaptive response for RCD is usually investigated by in vitro or in vivo clonogenic assay. To date, this has not been demonstrated in the vulval tissue in Caenorhabditis elegans (C. elegans), which is a well established in vivo model for radiation-induced RCD. In this study to determine whether radioadaptive response occurs in the vulval tissue model of C. elegans, early larval worms were gamma irradiated with lower adaptive doses, followed by higher challenge doses. The ratio of protruding vulva was used to assess the RCD of vulval cells. The results of this study showed that the radioadaptive response for RCD in this vulval tissue model could be well induced by dose combinations of 5 + 75 Gy and 5 + 100 Gy at the time point of 14-16 h in worm development. In addition, the time course analysis indicated that radioresistance in vulval cells developed within 1.75 h after an adaptive dose and persisted for only a short period of time (2-4 h). DNA damage checkpoint and non-homologous end joining were involved in the radioadaptive response, exhibiting induction of protruding vulva in worms deficient in these two pathways similar to their controls. Interestingly, the DNA damage checkpoint was not active in the somatic vulval cells, and it was therefore suggested that the DNA damage checkpoint might mediate the radioadaptive response in a cell nonautonomous manner. Here, we show evidence of the occurrence of a radioadaptive response for RCD in the vulval tissue model of C. elegans. This finding provides a potential opportunity to gain further insight into the underlying mechanisms of the radioadaptive response for RCD, in view of the abundant genetic resources of C. elegans. (C) 2016 by Radiation Research Society |
| WOS关键词 | DNA-DAMAGE RESPONSE ; ADAPTIVE RESPONSE ; STRESS RESISTANCE ; CLONOGENIC-ASSAY ; GERM-CELLS ; RADIATION ; CANCER ; APOPTOSIS ; HOMOLOG ; REPAIR |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000376752200008 |
| 资助机构 | NIH National Center for Research Resources ; NIH National Center for Research Resources ; NIH National Center for Research Resources ; NIH National Center for Research Resources ; National Natural Science Foundation of China(U1332127 ; National Natural Science Foundation of China(U1332127 ; National Natural Science Foundation of China(U1332127 ; National Natural Science Foundation of China(U1332127 ; 11275230 ; 11275230 ; 11275230 ; 11275230 ; 11575233) ; 11575233) ; 11575233) ; 11575233) ; NIH National Center for Research Resources ; NIH National Center for Research Resources ; NIH National Center for Research Resources ; NIH National Center for Research Resources ; National Natural Science Foundation of China(U1332127 ; National Natural Science Foundation of China(U1332127 ; National Natural Science Foundation of China(U1332127 ; National Natural Science Foundation of China(U1332127 ; 11275230 ; 11275230 ; 11275230 ; 11275230 ; 11575233) ; 11575233) ; 11575233) ; 11575233) |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/22423]  |
| 专题 | 合肥物质科学研究院_技术生物与农业工程研究所 |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China 2.Chinese Acad Sci, Key Lab Environm Toxicol & Pollut Control Technol, Inst Tech Biol & Agr Engn, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Huangqi,Chen, Liangwen,Liu, Jialu,et al. Radioadaptive Response for Reproductive Cell Death Demonstrated in In Vivo Tissue Model of Caenorhabditis elegans[J]. RADIATION RESEARCH,2016,185(4):402-410. |
| APA | Tang, Huangqi.,Chen, Liangwen.,Liu, Jialu.,Shi, Jue.,Li, Qingqing.,...&Bian, Po.(2016).Radioadaptive Response for Reproductive Cell Death Demonstrated in In Vivo Tissue Model of Caenorhabditis elegans.RADIATION RESEARCH,185(4),402-410. |
| MLA | Tang, Huangqi,et al."Radioadaptive Response for Reproductive Cell Death Demonstrated in In Vivo Tissue Model of Caenorhabditis elegans".RADIATION RESEARCH 185.4(2016):402-410. |
入库方式： OAI收割
来源：合肥物质科学研究院
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