中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 合肥物质科学研究院
  3. 中国科学院合肥物质科学研究院
  4. 技术生物与农业工程研究所
Investigating the environmental factors affecting the toxicity of silver nanoparticles in Escherichia coli with dual fluorescence analysis

文献类型：期刊论文

作者Hong, Wei1,2; Li, Luzhi1,2; Liang, Junting1,2; Wang, Jingjing1,2; Wang, Xuanyu1,2; Xu, Shengmin1,2; Wu, Lijun1,2; Zhao, Guoping1,2; Xu, An1,2; Chen, Shaopeng1,2
刊名CHEMOSPHERE
出版日期2016-07-01
卷号155期号:无页码:329-335
关键词Silver Nanoparticles Toxic Effects Dual Fluorescence Analysis Environmental Factor
DOI10.1016/j.chemosphere.2016.04.074
文献子类Article
英文摘要Flow cytometric investigation of the toxic effects of nanoparticles on bacteria is highly challenging and not sensitive due to the interference of aggregated nanoparticles: aggregated nanoparticles and bacteria are similar in size. In this study, an optimized dual fluorescence flow cytometric analysis was developed using PI-Lac::GFP (propidium iodide stained Escherichia coli (lac::GFP)) to monitor the toxicity of silver nanoparticles (AgNPs). As compared with single fluorescence analysis, the dual fluorescence analysis enabled more accurate evaluation of the toxic effects of AgNPs. We used this dual fluorescence analysis to investigate how AgNPs toxicity was affected by two typical environmental factors, divalent metal ions and surfactants. Our data revealed that Cu2+ and SDS significantly enhanced the toxicity of AgNPs in a dose-dependent manner. SDS enhanced the toxicity of both AgNPs and Ag+ ions, whereas Cu2+ increased the toxicity of AgNPs but not dissolved Ag+ ions. Our results suggest that this dual fluorescence analysis can be used to evaluate the toxicity of AgNPs accurately and sensitively. (C) 2016 Elsevier Ltd. All rights reserved.
WOS关键词ANTIBACTERIAL ACTIVITY ; FLOW-CYTOMETRY ; E. COLI ; STABILITY ; MECHANISM ; AGGREGATION ; DISSOLUTION ; INHIBITION ; PARTICLES ; SURFACES
WOS研究方向Environmental Sciences & Ecology
语种英语
WOS记录号WOS:000377736100039
资助机构Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Major National Scientific Research Projects(2014CB932002) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Strategic Leading Science & Technology Program (B)(XDB14030502) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2014FXCX010) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CASHIPS director's fund(YZJJ201501) ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams ; CAS/SAFEA International Partnership Program for Creative Research Teams
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/22455]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei, Anhui, Peoples R China
2.Key Lab Environm Toxicol & Pollut Control Technol, Hefei, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Hong, Wei,Li, Luzhi,Liang, Junting,et al. Investigating the environmental factors affecting the toxicity of silver nanoparticles in Escherichia coli with dual fluorescence analysis[J]. CHEMOSPHERE,2016,155(无):329-335.
APA Hong, Wei.,Li, Luzhi.,Liang, Junting.,Wang, Jingjing.,Wang, Xuanyu.,...&Chen, Shaopeng.(2016).Investigating the environmental factors affecting the toxicity of silver nanoparticles in Escherichia coli with dual fluorescence analysis.CHEMOSPHERE,155(无),329-335.
MLA Hong, Wei,et al."Investigating the environmental factors affecting the toxicity of silver nanoparticles in Escherichia coli with dual fluorescence analysis".CHEMOSPHERE 155.无(2016):329-335.

入库方式： OAI收割

来源：合肥物质科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。