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A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide

文献类型：期刊论文

作者Ke, Zhigang1; Ma, Shanshan1; Li, Lamei1; Huang, Qing1,2
刊名CHEMICAL PHYSICS LETTERS
出版日期2016-07-16
卷号657期号:无页码:49-52
关键词Fluorescence Horseradish Peroxidase Protein Unfolding Heme
DOI10.1016/j.cplett.2016.05.055
文献子类Article
英文摘要Horseradish peroxidase (HRP) is a classical heme-containing protein which has been applied in many fields. The prosthetic group heme in HRP, especially in unfolded state, can react with hydrogen peroxide (H2O2) to produce a fluorescent product with the maximum emission wavelength at 450 nm. Utilizing this emission band as a fluorescence probe, the unfolding process of HRP in urea can be assessed quantitatively, and the calculated thermodynamic parameters are consistent with those determined by circular dichroism (CD) at 222 nm and steady-state tryptophan (Trp) fluorescence methods. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词NUCLEAR-MAGNETIC-RESONANCE ; X-RAY-SCATTERING ; CIRCULAR-DICHROISM ; GUANIDINE-HYDROCHLORIDE ; CYTOCHROME-C ; PROTEINS ; DENATURATION ; SPECTROSCOPY ; INACTIVATION ; STABILITY
WOS研究方向Chemistry ; Physics
语种英语
WOS记录号WOS:000383444700009
资助机构Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; 11175204 ; 11175204 ; 11175204 ; 11175204 ; 11475217 ; 11475217 ; 11475217 ; 11475217 ; 11405220) ; 11405220) ; 11405220) ; 11405220) ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; 11175204 ; 11175204 ; 11175204 ; 11175204 ; 11475217 ; 11475217 ; 11475217 ; 11475217 ; 11405220) ; 11405220) ; 11405220) ; 11405220)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/24255]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China
2.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714		 Ke, Zhigang,Ma, Shanshan,Li, Lamei,et al. A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide[J]. CHEMICAL PHYSICS LETTERS,2016,657(无):49-52.
APA Ke, Zhigang,Ma, Shanshan,Li, Lamei,&Huang, Qing.(2016).A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide.CHEMICAL PHYSICS LETTERS,657(无),49-52.
MLA Ke, Zhigang,et al."A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide".CHEMICAL PHYSICS LETTERS 657.无(2016):49-52.

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来源：合肥物质科学研究院

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