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Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy

文献类型：期刊论文

作者Liu, Jinghua1,2; Huang, Qing1,2,3
刊名APPLIED SPECTROSCOPY
出版日期2016-10-01
卷号70期号:10页码:1639-1648
关键词Astaxanthin Fourier Transform Infrared Ft-ir Microspectroscopy Haematococcus Pluvialis Raman Microspectroscopy
DOI10.1177/0003702816645605
文献子类Article
英文摘要Haematococcus pluvialis has promising applications owing to its ability to accumulate astaxanthin under stress conditions. In order to acquire higher astaxanthin productivity from H. pluvialis, it is critical not only to develop efficient mutagenesis techniques, but also to establish rapid and effective screening methods which are highly demanded in current research and application practice. In this work, we therefore attempted to develop a new approach to screening the astaxanthin-hyperproducing strains based on spectroscopic tools. Using Fourier transform infrared (FT-IR) and Raman microspectroscopy, we have achieved rapid and quantitative analysis of the algal cells in terms of astaxanthin, beta-carotene, proteins, lipids, and carbohydrates. In particular, we have found that the ratio of the IR absorption band at 1740 cm(-1) to the band at 1156 cm(-1) can be utilized for identifying astaxanthin-hyperproducing strains. This work may therefore open a new avenue for developing high-throughput screening methods necessary for the microbial mutant breeding industry.
WOS关键词MULTIVARIATE CURVE RESOLUTION ; CAROTENOGENIC GENES ; YEAST-CELL ; SPECTROSCOPY ; MICROALGAE ; MICROSCOPY ; SCATTERING ; MUTANTS ; STRESS ; ACCUMULATION
WOS研究方向Instruments & Instrumentation ; Spectroscopy
语种英语
WOS记录号WOS:000386026300003
资助机构National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/30143]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei, Peoples R China
3.Univ Sci & Technol China, Sch Nucl Sci & Technol, Hefei, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Jinghua,Huang, Qing. Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy[J]. APPLIED SPECTROSCOPY,2016,70(10):1639-1648.
APA Liu, Jinghua,&Huang, Qing.(2016).Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy.APPLIED SPECTROSCOPY,70(10),1639-1648.
MLA Liu, Jinghua,et al."Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy".APPLIED SPECTROSCOPY 70.10(2016):1639-1648.

入库方式： OAI收割

来源：合肥物质科学研究院

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