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  4. 技术生物与农业工程研究所
In vivo synthesis of nano-selenium by Tetrahymena thermophila SB210

文献类型：期刊论文

作者Cui, Yin-Hua1; Li, Ling-Li1; Zhou, Nan-Qing1; Liu, Jing-Hua2; Huang, Qing2; Wang, Hui-Juan3; Tian, Jie3; Yu, Han-Qing1
刊名ENZYME AND MICROBIAL TECHNOLOGY
出版日期2016-12-01
卷号95期号:无页码:185-191
关键词Tetrahymena Thermophila Nano-selenium Animal Biosynthesis Glutathione (Gsh) Proteins
DOI10.1016/j.enzmictec.2016.08.017
文献子类Article
英文摘要Nano-selenium has a great potential to be used in chemical, biological, medical and environmental fields. Biological methods for nano-selenium synthesis have attracted wide interests, because they can be operated at ambient temperature and pressure without complicated equipments. In this work, a protozoa, Tetrahymena thermophila (T. thermophila) SB210, was used to in vivo synthesize nano-selenium. The biosynthesized nano-selenium was characterized using transmission electron microscopy, energy dispersive X-ray spectroscopy and Raman spectroscopy. The synthesized amorphous spherical selenium nanoparticles had diameters of 50-500 nm with the coexistence of irregular nano-selenium. The expressions of glutathione (GSH) synthesis related gene glutathione synthase, cysteine-rich protein metallothionein related gene metallothionein-1 and [2Fe-2S] cluster-binding protein related gene were up-regulated in the nano-selenium producing group. Also, the subsequent GSH detection and in vitro synthesis experimental results suggest the three proteins were likely to be involved in the nano-selenium synthesis process. (C) 2016 Elsevier Inc. All rights reserved.
WOS关键词ELEMENTAL SELENIUM ; AMORPHOUS SELENIUM ; NANOPARTICLES ; GLUTATHIONE ; REDUCTION ; ANTIOXIDANT ; METABOLISM ; MECHANISM ; CELLS
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000389161400021
资助机构Natural Science Foundation of China(21477120) ; Natural Science Foundation of China(21477120) ; Natural Science Foundation of China(21477120) ; Natural Science Foundation of China(21477120) ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Natural Science Foundation of China(21477120) ; Natural Science Foundation of China(21477120) ; Natural Science Foundation of China(21477120) ; Natural Science Foundation of China(21477120) ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/30250]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.CAS Key Lab Urban Pollutant Convers, Dept Chem, Shanghai, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China
3.Univ Sci & Technol China, Engn & Mat Sci Expt Ctr, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714		 Cui, Yin-Hua,Li, Ling-Li,Zhou, Nan-Qing,et al. In vivo synthesis of nano-selenium by Tetrahymena thermophila SB210[J]. ENZYME AND MICROBIAL TECHNOLOGY,2016,95(无):185-191.
APA Cui, Yin-Hua.,Li, Ling-Li.,Zhou, Nan-Qing.,Liu, Jing-Hua.,Huang, Qing.,...&Yu, Han-Qing.(2016).In vivo synthesis of nano-selenium by Tetrahymena thermophila SB210.ENZYME AND MICROBIAL TECHNOLOGY,95(无),185-191.
MLA Cui, Yin-Hua,et al."In vivo synthesis of nano-selenium by Tetrahymena thermophila SB210".ENZYME AND MICROBIAL TECHNOLOGY 95.无(2016):185-191.

入库方式： OAI收割

来源：合肥物质科学研究院

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