中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 合肥物质科学研究院
  3. 中国科学院合肥物质科学研究院
  4. 技术生物与农业工程研究所
A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans

文献类型：期刊论文

作者Luo, Xun1,2,4; Xu, Shengmin1,3; Yang, Yaning1,3; Zhang, Yajun1,3; Wang, Shunchang4; Chen, Shaopeng1,3; Xu, An1,3; Wu, Lijun1,2,3
刊名CHEMOSPHERE
出版日期2017-02-01
卷号168期号:无页码:648-657
关键词Ngg Agnps Toxicity Germ Cell Apoptosis C. Elegans Nanotoxicity
DOI10.1016/j.chemosphere.2016.11.011
文献子类Article
英文摘要At present, nanotechnology has been producing nanoscale materials with unprecedented speed. Nano materials could be inevitably released into the environment owing to their widespread use, and their potential toxicity has caused a great concern. With regard to assessment of nanomaterial toxicity, many studies probably don't truly reflect their toxicity, because the nanoparticles were not stable and uniformly dispersed in the medium. In the present study, the semi-fluid nematode growth gelrite medium (NGG) was used to achieve better distribution of silver nanoparticles (AgNPs). We aimed to evaluate the toxicity of AgNPs in three different culture methods, such as the NGG, nematode growth medium (NGM) and K-medium (KM). Our transmission electron microscopy, hydrodynamic diameter, and inductively coupled plasma-atomic emission spectrometry results demonstrated that AgNPs homogeneously and stably dispersed in NGG compared to that in liquid KM. Furthermore, the conventional toxicity end points, such as body length, fecundity, lifespan, population growth, germline cell apoptosis, reactive oxygen species, and mitochondrial membrane potential were used to assess the toxicity of AgNPs to Caenorhabditis elegans (C. elegans) in NGG, NGM and KM. Our results showed that the toxicity of AgNPs obtained in the NGG test medium was much higher than that in the standard NGM and KM. In addition to the improved dispersion of nanoparticles, NGG also offered advantages for long-term studies and likely provided a convenient nematode toxicity testing method. These results revealed that the NGG test medium was a suitable and sensitive culture method for the evaluation of AgNPs toxicity using C elegans. (C) 2016 Elsevier Ltd. All rights reserved.
WOS关键词OXIDATIVE STRESS ; GRAPHENE OXIDE ; DNA-DAMAGE ; NEMATODE ; GROWTH ; TOXICOLOGY ; APOPTOSIS ; EXPOSURE ; BEHAVIOR ; CELLS
WOS研究方向Environmental Sciences & Ecology
语种英语
WOS记录号WOS:000391897500078
资助机构National Basic Research 973 Program(2014CB932002) ; National Basic Research 973 Program(2014CB932002) ; National Basic Research 973 Program(2014CB932002) ; National Basic Research 973 Program(2014CB932002) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; National Natural Science Foundation of China(81301182) ; National Natural Science Foundation of China(81301182) ; National Natural Science Foundation of China(81301182) ; National Natural Science Foundation of China(81301182) ; National Basic Research 973 Program(2014CB932002) ; National Basic Research 973 Program(2014CB932002) ; National Basic Research 973 Program(2014CB932002) ; National Basic Research 973 Program(2014CB932002) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030502) ; National Natural Science Foundation of China(81301182) ; National Natural Science Foundation of China(81301182) ; National Natural Science Foundation of China(81301182) ; National Natural Science Foundation of China(81301182)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/32789]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.Chinese Acad Sci, Key Lab Ion Beam Bioengn, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
3.Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Anhui, Peoples R China
4.Huainan Normal Univ, Sch Bioengn, Huainan 232038, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Luo, Xun,Xu, Shengmin,Yang, Yaning,et al. A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans[J]. CHEMOSPHERE,2017,168(无):648-657.
APA Luo, Xun.,Xu, Shengmin.,Yang, Yaning.,Zhang, Yajun.,Wang, Shunchang.,...&Wu, Lijun.(2017).A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans.CHEMOSPHERE,168(无),648-657.
MLA Luo, Xun,et al."A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans".CHEMOSPHERE 168.无(2017):648-657.

入库方式： OAI收割

来源：合肥物质科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。