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The Protein Neddylation Pathway in Trypanosoma brucei FUNCTIONAL CHARACTERIZATION AND SUBSTRATE IDENTIFICATION

文献类型：期刊论文

作者Liao, Shanhui1,2; Hu, Huiqing2; Wang, Tao2,3; Tu, Xiaoming1; Li, Ziyin2
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
出版日期2017-01-20
卷号292期号:3页码:1081-+
DOI10.1074/jbc.M116.766741
文献子类Article
英文摘要Protein posttranslational modifications such as neddylation play crucial roles in regulating protein function. Only a few neddylated substrates have been validated to date, and the role of neddylation remains poorly understood. Here, using Trypanosoma brucei as the model organism, we investigated the function and substrates of TbNedd8. TbNedd8 is distributed throughout the cytosol but enriched in the nucleus and the flagellum. Depletion of TbNedd8 by RNAi abolished global protein ubiquitination, caused DNA re-replication in postmitotic cells, impaired spindle assembly, and compromised the flagellum attachment zone filament, leading to flagellum detachment. Through affinity purification and mass spectrometry, we identified 70 TbNedd8-conjugated and -associated proteins, including known Nedd8-conjugated and -associated proteins, putative TbNedd8 conjugation system enzymes, proteins of diverse biological functions, and proteins of unknown function. Finally, we validated six Cullins as bona fide TbNedd8 substrates and identified the TbNedd8 conjugation site in three Cullins. This work lays the foundation for understanding the roles of protein neddylation in this early divergent parasitic protozoan.
WOS关键词ATTACHMENT ZONE FILAMENT ; UBIQUITIN-LIKE PROTEIN ; FLAGELLUM ATTACHMENT ; CELL-CYCLE ; CENTROSOME DUPLICATION ; MODIFIER NEDD8 ; IN-VIVO ; COMPLEX ; LIGASE ; CDT1
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000392318700027
资助机构National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 31500601) ; 31500601) ; 31500601) ; 31500601) ; 31500601) ; 31500601) ; 31500601) ; 31500601) ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; National Natural Science Foundation of China(31270780 ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; Fundamental Research Funds for the Central Universities(WK2070000020) ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; National Institutes of Health(R01AI101437 ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; Chinese Scholarship Council ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; U1332137 ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; R01AI118736) ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 21272090 ; 31500601) ; 31500601) ; 31500601) ; 31500601) ; 31500601) ; 31500601) ; 31500601) ; 31500601)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/32794]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
2.Univ Texas Hlth Sci Ctr Houston, McGovern Med Sch, Dept Microbiol & Mol Genet, Houston, TX 77030 USA
3.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Liao, Shanhui,Hu, Huiqing,Wang, Tao,et al. The Protein Neddylation Pathway in Trypanosoma brucei FUNCTIONAL CHARACTERIZATION AND SUBSTRATE IDENTIFICATION[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2017,292(3):1081-+.
APA Liao, Shanhui,Hu, Huiqing,Wang, Tao,Tu, Xiaoming,&Li, Ziyin.(2017).The Protein Neddylation Pathway in Trypanosoma brucei FUNCTIONAL CHARACTERIZATION AND SUBSTRATE IDENTIFICATION.JOURNAL OF BIOLOGICAL CHEMISTRY,292(3),1081-+.
MLA Liao, Shanhui,et al."The Protein Neddylation Pathway in Trypanosoma brucei FUNCTIONAL CHARACTERIZATION AND SUBSTRATE IDENTIFICATION".JOURNAL OF BIOLOGICAL CHEMISTRY 292.3(2017):1081-+.

入库方式： OAI收割

来源：合肥物质科学研究院

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