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Chemical Synthesis of K34-Ubiquitylated H2B for Nucleosome Reconstitution and Single-Particle Cryo-Electron Microscopy Structural Analysis

文献类型：期刊论文

作者Li, Jiabin1,2,3,4,5; He, Qiaoqiao1,2; Liu, Yuntao3,4,5; Liu, Sanling3,4,5; Tang, Shan1,2; Li, Chengmin6; Sun, Demeng3,4,5; Li, Xiaorun3,4,5; Zhou, Min6; Zhu, Ping6
刊名CHEMBIOCHEM
出版日期2017-01-17
卷号18期号:2页码:176-180
关键词Cryo-electron Microscopy Histone Nucleosome Protein Chemical Synthesis Ubiquitylation
DOI10.1002/cbic.201600551
文献子类Article
英文摘要Post-translational modifications (e.g., ubiquitylation) of histones play important roles in dynamic regulation of chromatin. Histone ubiquitylation has been speculated to directly influence the structure and dynamics of nucleosomes. However, structural information for ubiquitylated nucleosomes is still lacking. Here we report an alternative strategy for total chemical synthesis of homogenous histone H2B-K34-ubiquitylation (H2B-K34Ub) by using acid-cleavable auxiliary-mediated ligation of peptide hydrazides for site-specific ubiquitylation. Synthetic H2B-K34Ub was efficiently incorporated into nucleosomes and further used for single-particle cryo-electron microscopy (cryo-EM) imaging. The cryo-EM structure of the nucleosome containing H2B-K34Ub suggests that two flexible ubiquitin domains protrude between the DNA chains of the nucleosomes. The DNA chains around the H2B-K34 sites shift and provide more space for ubiquitin to protrude. These analyses indicated local and slight structural influences on the nucleosome with ubiquitylation at the H2B-K34 site.
WOS关键词HISTONE UBIQUITYLATION ; PEPTIDE HYDRAZIDES ; H3 K4 ; METHYLATION ; PROTEINS ; LIGATION ; UBIQUITINATION ; DEUBIQUITINASES ; SEMISYNTHESIS ; AUXILIARIES
WOS研究方向Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000392936800005
资助机构National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; 21621003) ; 21621003) ; 21621003) ; 21621003) ; 21621003) ; 21621003) ; 21621003) ; 21621003) ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; National Natural Science Foundation of China(21532004 ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Key Research Plan-Protein Science(2015CB910100) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Chinese Academy of Sciences (CAS)(XDB08030302) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; Fundamental Research Funds for the Central Universities of China(WK2340000064) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; CAS-Hefei Science Center grant(2015HSC-SRG051) ; 21621003) ; 21621003) ; 21621003) ; 21621003) ; 21621003) ; 21621003) ; 21621003) ; 21621003)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/32818]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Bioorgan Phosphorus Chem Chem Biol, Beijing 100084, Peoples R China
2.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
3.Univ Sci & Technol China, Hefei Natl Lab Phys Sci MicroScale, Hefei 230027, Peoples R China
4.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
5.Chinese Acad Sci, High Field Magnet Lab, Hefei 230027, Peoples R China
6.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
7.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
8.Univ Calif Los Angeles, Calif NanoSyst Syst, Los Angeles, CA 90095 USA
推荐引用方式
GB/T 7714		 Li, Jiabin,He, Qiaoqiao,Liu, Yuntao,et al. Chemical Synthesis of K34-Ubiquitylated H2B for Nucleosome Reconstitution and Single-Particle Cryo-Electron Microscopy Structural Analysis[J]. CHEMBIOCHEM,2017,18(2):176-180.
APA Li, Jiabin.,He, Qiaoqiao.,Liu, Yuntao.,Liu, Sanling.,Tang, Shan.,...&Tian, Changlin.(2017).Chemical Synthesis of K34-Ubiquitylated H2B for Nucleosome Reconstitution and Single-Particle Cryo-Electron Microscopy Structural Analysis.CHEMBIOCHEM,18(2),176-180.
MLA Li, Jiabin,et al."Chemical Synthesis of K34-Ubiquitylated H2B for Nucleosome Reconstitution and Single-Particle Cryo-Electron Microscopy Structural Analysis".CHEMBIOCHEM 18.2(2017):176-180.

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来源：合肥物质科学研究院

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