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The change of the state of cell membrane can enhance the synthesis of menaquinone in Escherichia coli

文献类型：期刊论文

作者Liu, Yan1,2,3; Ding, Xiu-min1; Xue, Zheng-lian1; Hu, Liu-xiu1; Zhang, Ning-juan1; Wang, Zhou1; Yang, Jian-wei1; Cheng, Qian1; Chen, Ming-hong1; Zhang, Zhuang-zhuang3
刊名WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
出版日期2017-03-01
卷号33期号:3页码:1-11
关键词Menaquinone Cell Membranes Membrane Fluidity Leakage
DOI10.1007/s11274-017-2222-9
文献子类Article
英文摘要Menaquinone (MK) was an attractive membrane-bound intracellular chemical. To enhance its production, we tried to find the relationship between its synthesis and the state of cell membrane in producing strain. Due to non-ionic surfactant-polyoxyethylene oleyl ether (POE) and plant oil-cedar wood oil (CWO) can typically increase extracellular secretion and intracellular synthesis of MK respectively, the effect of these two substances on cell morphology, physical properties of cell membrane was investigated. Finally, two engineering strains were constructed to verify whether the state of cell membrane can enhance MK synthesis. The result showed that the edge of cells was broken when POE added in the medium. Other physical properties such as total fatty acid content decreased by 40.7% and the ratio of saturated fatty acids to unsaturated fatty acids decreased from 1.58 +/- 0.05 to 1.31 +/- 0.04. Mean-while, cell membrane leakage was enhanced from 7.14 to 64.31%. Different from POE group, cell membrane was intact in CWO group. Moreover, the ratio of saturated fatty acids to unsaturated fatty acids increased from 1.58 +/- 0.05 to 1.78 +/- 0.04 and the average lipid length decreased from 16.05 +/- 0.08 to 15.99 +/- 0.10. Two constructed strains, especially Escherichia coli DH5 alpha FatB, exhibited strong MK secretion ability and the extracellular MK reached 10.71 +/- 0.19 mg/L. An understanding of these functionary mechanisms could not only provide a new idea for the synthesis of MK, but also provide a reference to increase the yield of intracellular membrane-bound metabolites.
WOS关键词FREE FATTY-ACIDS ; VITAMIN-K-2 ; SUPPLEMENTATION ; THIOESTERASE ; PATHWAY ; MODEL
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000398100300011
资助机构National Nature Science Foundation of China(31501465 ; National Nature Science Foundation of China(31501465 ; National Nature Science Foundation of China(31501465 ; National Nature Science Foundation of China(31501465 ; National High-tech RD Program(2014AA021704) ; National High-tech RD Program(2014AA021704) ; National High-tech RD Program(2014AA021704) ; National High-tech RD Program(2014AA021704) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; 31471615) ; 31471615) ; 31471615) ; 31471615) ; National Nature Science Foundation of China(31501465 ; National Nature Science Foundation of China(31501465 ; National Nature Science Foundation of China(31501465 ; National Nature Science Foundation of China(31501465 ; National High-tech RD Program(2014AA021704) ; National High-tech RD Program(2014AA021704) ; National High-tech RD Program(2014AA021704) ; National High-tech RD Program(2014AA021704) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Provincial Nature Science Foundation(1608085QC54) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Higher Education Exchange Program for Young Talents(gxfxZD2016109) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; Anhui Provincial Undergraduate Innovation and Entrepreneurship Program(201510363130) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; National Undergraduate Innovation and Entrepreneurship Program(2016103630055) ; 31471615) ; 31471615) ; 31471615) ; 31471615)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/32899]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.Anhui Polytech Univ, Coll Biochem Engn, Wuhu 241000, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China
3.Virginia Commonwealth Univ, Sch Med, VCU Massey Canc Ctr, VCU Inst Mol Genet, Med Coll Virginia Campus, Richmond, VA 23298 USA
推荐引用方式
GB/T 7714		 Liu, Yan,Ding, Xiu-min,Xue, Zheng-lian,et al. The change of the state of cell membrane can enhance the synthesis of menaquinone in Escherichia coli[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2017,33(3):1-11.
APA Liu, Yan.,Ding, Xiu-min.,Xue, Zheng-lian.,Hu, Liu-xiu.,Zhang, Ning-juan.,...&Zheng, Zhi-ming.(2017).The change of the state of cell membrane can enhance the synthesis of menaquinone in Escherichia coli.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,33(3),1-11.
MLA Liu, Yan,et al."The change of the state of cell membrane can enhance the synthesis of menaquinone in Escherichia coli".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 33.3(2017):1-11.

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来源：合肥物质科学研究院

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