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  4. 中科院强磁场科学中心
27 T ultra-high static magnetic field changes orientation and morphology of mitotic spindles in human cells

文献类型：期刊论文

作者Zhang, Lei1,2; Hou, Yubin1; Li, Zhiyuan1; Ji, Xinmiao1; Wang, Ze1,2; Wang, Huizhen1,2; Tian, Xiaofei1,2; Yu, Fazhi2; Yang, Zhenye2; Pi, Li1,2
刊名ELIFE
出版日期2017-02-28
卷号6期号:无页码:1-21
DOI10.7554/eLife.22911.001
文献子类Article
英文摘要Purified microtubules have been shown to align along the static magnetic field (SMF) in vitro because of their diamagnetic anisotropy. However, whether mitotic spindle in cells can be aligned by magnetic field has not been experimentally proved. In particular, the biological effects of SMF of above 20 T (Tesla) have never been reported. Here we found that in both CNE-2Z and RPE1 human cells spindle orients in 27 T SMF. The direction of spindle alignment depended on the extent to which chromosomes were aligned to form a planar metaphase plate. Our results show that the magnetic torque acts on both microtubules and chromosomes, and the preferred direction of spindle alignment relative to the field depends more on chromosome alignment than microtubules. In addition, spindle morphology was also perturbed by 27 T SMF. This is the first reported study that investigated the cellular responses to ultra-high magnetic field of above 20 T. Our study not only found that ultra-high magnetic field can change the orientation and morphology of mitotic spindles, but also provided a tool to probe the role of spindle orientation and perturbation in developmental and cancer biology.
WOS关键词DIAMAGNETIC ANISOTROPY ; BULL SPERMS ; FROG EGGS ; MICROTUBULES ; SUBSTRATUM ; MIGRATION ; ALIGNMENT ; DYNAMICS ; EXPOSURE ; DIVISION
WOS研究方向Life Sciences & Biomedicine - Other Topics
语种英语
WOS记录号WOS:000397652000001
资助机构National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Key Research and Development Program of China(2016YFA0400900) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; National Natural Science Foundation of China(U1532151) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007) ; Hefei Science Center(2016HSC-1U007)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/32922]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Chinese Acad Sci, High Field Magnet Lab, Hefei, Peoples R China
2.Univ Sci & Technol China, Hefei, Peoples R China
3.Harvard Med Sch, Dept Syst Biol, Boston, MA 02215 USA
4.Nanjing Univ, Collaborat Innovat Ctr Adv Microstruct, Nanjing, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Lei,Hou, Yubin,Li, Zhiyuan,et al. 27 T ultra-high static magnetic field changes orientation and morphology of mitotic spindles in human cells[J]. ELIFE,2017,6(无):1-21.
APA Zhang, Lei.,Hou, Yubin.,Li, Zhiyuan.,Ji, Xinmiao.,Wang, Ze.,...&Zhang, Xin.(2017).27 T ultra-high static magnetic field changes orientation and morphology of mitotic spindles in human cells.ELIFE,6(无),1-21.
MLA Zhang, Lei,et al."27 T ultra-high static magnetic field changes orientation and morphology of mitotic spindles in human cells".ELIFE 6.无(2017):1-21.

入库方式： OAI收割

来源：合肥物质科学研究院

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