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Backbone and side-chain assignments for a novel CBM69 starch binding domain AmyP-SBD

文献类型：期刊论文

作者Li, Xinxin1; Yu, Jigang1; Zhang, Jiahai2; Sun, Hongbin3; Zhang, Xuecheng1
刊名BIOMOLECULAR NMR ASSIGNMENTS
出版日期2017-10-01
卷号11期号:2页码:235-237
关键词Starch Binding Domain Cbm69 Amyp Nmr
DOI10.1007/s12104-017-9755-6
文献子类Article
英文摘要Starch binding domains (SBDs) are important for the functions of glycoside hydrolysis enzymes such as alpha-amylases, they have great application potential in biotechnology and industries. AmyP is a newly identified alpha-amylase belonging to a new subfamily 37 of glycoside hydrolysis enzyme family 13. AmyP shows preferential degradation to soluble starch, in which its C-terminal starch binding domain, AmyP-SBD, plays an important role. AmyP-SBD shares very low sequence similarity with other biochemically characterized SBDs and was assigned to a new carbohydrate binding module family CBM69. Intriguingly, AmyP-SBD is unfolded in free form, and substrate analogue beta-cyclodextrin may induce it to fold into a relatively rigid state. Structure determination for AmyP-SBD will be helpful for understanding its unique properties. Here, we report the backbone and side-chain H-1, C-13 and N-15 resonance assignments of folded AmyP-SBD, as a basis for structure determination and further studies.
WOS关键词ALPHA-AMYLASE ENZYMES ; ASPERGILLUS-NIGER ; GLUCOAMYLASE ; FAMILY
WOS研究方向Biophysics ; Spectroscopy
语种英语
WOS记录号WOS:000410473300023
资助机构National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; National Natural Science Foundation of China(31470775) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010) ; International Science and Technology Cooperation Plan of Anhui Province(1503062010)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/33669]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Anhui Univ, Sch Life Sci, Anhui Prov Engn Technol Res Ctr Microorganisms &, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China
3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Li, Xinxin,Yu, Jigang,Zhang, Jiahai,et al. Backbone and side-chain assignments for a novel CBM69 starch binding domain AmyP-SBD[J]. BIOMOLECULAR NMR ASSIGNMENTS,2017,11(2):235-237.
APA Li, Xinxin,Yu, Jigang,Zhang, Jiahai,Sun, Hongbin,&Zhang, Xuecheng.(2017).Backbone and side-chain assignments for a novel CBM69 starch binding domain AmyP-SBD.BIOMOLECULAR NMR ASSIGNMENTS,11(2),235-237.
MLA Li, Xinxin,et al."Backbone and side-chain assignments for a novel CBM69 starch binding domain AmyP-SBD".BIOMOLECULAR NMR ASSIGNMENTS 11.2(2017):235-237.

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来源：合肥物质科学研究院

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