中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 合肥物质科学研究院
  3. 中国科学院合肥物质科学研究院
  4. 中科院强磁场科学中心
Improved chemical synthesis of o-nirtrobenzyl-tyrosine for concise site-specific N-15-tyrosine NMR analysis demonstrated by plant ABA receptor PYL10

文献类型：期刊论文

作者Guo, Xiaoqi1,2,3; Zhang, Bo1,2; He, Yao1,2; Liu, Yangzhong3; Tian, Changlin1,2
刊名TETRAHEDRON LETTERS
出版日期2017-09-27
卷号58期号:39页码:3764-3767
关键词Unnatural Amino Acid Tyrosine Protein Nmr Analysis Abscisic Acid Receptor Pyl-10
DOI10.1016/j.tetlet.2017.08.034
文献子类Article
英文摘要The yield of unnatural amino acid o-nitrobenzyl-tyrosine (oNBTyr), which was able to recover to natural tyrosine after UV-photocleavage was greatly improved from 20% to 81% by using 2-nitrobenzyl bromide as the nucleophilic reagent. Through genetically introducing N-15-oNBY and consequent photo-cleavage, the site-specific N-15-Tyr NMR analysis of plant ABA (abscisic acid) receptor PYL10 was implemented without any residue variation. This isotope labelling of tyrosine onto protein backbone provides a convenient strategy for NMR analysis. (C) 2017 Published by Elsevier Ltd.
WOS关键词ABSCISIC-ACID ; PROTEIN ; DYNAMICS ; SPECTROSCOPY ; ARABIDOPSIS ; BACKBONE ; COMPLEX ; PROBE
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000412041400003
资助机构Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; U1432136) ; U1432136) ; U1432136) ; U1432136) ; U1432136) ; U1432136) ; U1432136) ; U1432136) ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Key Research Plan Protein Science(2015CB910100 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; Chinese Natural Science Foundation(U1332138 ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; 2016YFA0400900) ; U1432136) ; U1432136) ; U1432136) ; U1432136) ; U1432136) ; U1432136) ; U1432136) ; U1432136)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/33764]  
专题合肥物质科学研究院_中科院强磁场科学中心
作者单位1.Chinese Acad Sci, High Magnet Field Lab, Hefei, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei Nat Lab Phys Sci Microscale, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
3.Univ Sci & Technol China, Sch Chem & Mat Sci, Hefei 230026, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Guo, Xiaoqi,Zhang, Bo,He, Yao,et al. Improved chemical synthesis of o-nirtrobenzyl-tyrosine for concise site-specific N-15-tyrosine NMR analysis demonstrated by plant ABA receptor PYL10[J]. TETRAHEDRON LETTERS,2017,58(39):3764-3767.
APA Guo, Xiaoqi,Zhang, Bo,He, Yao,Liu, Yangzhong,&Tian, Changlin.(2017).Improved chemical synthesis of o-nirtrobenzyl-tyrosine for concise site-specific N-15-tyrosine NMR analysis demonstrated by plant ABA receptor PYL10.TETRAHEDRON LETTERS,58(39),3764-3767.
MLA Guo, Xiaoqi,et al."Improved chemical synthesis of o-nirtrobenzyl-tyrosine for concise site-specific N-15-tyrosine NMR analysis demonstrated by plant ABA receptor PYL10".TETRAHEDRON LETTERS 58.39(2017):3764-3767.

入库方式： OAI收割

来源：合肥物质科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。