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Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level

文献类型：期刊论文

作者Chen, Xiangyu1,2; Liang, Zhiting1; Li, Daobo3; Xiong, Ying1; Xiong, Penghui1; Guan, Yong1; Hou, Shuangyue1; Hu, Yue1; Chen, Shan1; Liu, Gang1
刊名BIOSENSORS & BIOELECTRONICS
出版日期2018-01-15
卷号99期号:无页码:416-423
关键词Microfluidic Chip Dielectrophoresis Shewanella Oneidensis Fluorescence Cell Level
DOI10.1016/j.bios.2017.08.017
文献子类Article
英文摘要Shewanella oneidensis, a model organism for electrochemical activity bacteria, has been widely studied at the biofilm level. However, to obtain more information regarding this species, it is essential to develop an approach to trap and detect S. oneidensis at the cell level. In this study, we report a rapid and label-free microfluidic platform for trapping, counting and detecting S. oneidensis cells. A microfluidic chip was integrated with a modified dielectrophoresis (DEP) trapping technique and hole arrays of different hole sizes. By numerical simulation and an elaborate electric field distribution design, S. oneidensis cells were successfully trapped and positioned in the hole arrays. Real time fluorescence imaging was also used to observe the trapping process. With the aid of a homemade image program, the trapped bacteria were accurately counted, and the results demonstrated that the amount of bacteria correlated with the hole sizes. As one of the significant applications of the device, Raman identification and detection of countable S. oneidensis cells was accomplished in two kinds of holes. The microfluidic platform provides a quantitative sample preparation and analysis method at the cell level that could be widely applied in the environmental and energy fields.
WOS关键词LABEL-FREE DETECTION ; ESCHERICHIA-COLI ; TECHNOLOGY ; MR-1 ; BIODECOLORIZATION ; SEPARATION ; BACTERIA ; WATER ; NOV
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000413284200056
资助机构National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123)
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/33813]  
专题合肥物质科学研究院_中科院合肥智能机械研究所
作者单位1.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China
2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
3.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Xiangyu,Liang, Zhiting,Li, Daobo,et al. Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level[J]. BIOSENSORS & BIOELECTRONICS,2018,99(无):416-423.
APA Chen, Xiangyu.,Liang, Zhiting.,Li, Daobo.,Xiong, Ying.,Xiong, Penghui.,...&Tian, Yangchao.(2018).Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level.BIOSENSORS & BIOELECTRONICS,99(无),416-423.
MLA Chen, Xiangyu,et al."Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level".BIOSENSORS & BIOELECTRONICS 99.无(2018):416-423.

入库方式： OAI收割

来源：合肥物质科学研究院

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