Rapid and Noninvasive Detection of Skin Cholesterol with Diffuse Reflectance Spectroscopy Technology
文献类型：期刊论文
| 作者 | Hou Hua-yi1,2; Dong Mei-li1,3 ; Wang Yi-kun1,3; Zhu Ling1,3; Ma Zu-chang1; Liu Yong1,3
|
| 刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 36期号:10页码:3215-3221 |
| 关键词 | Skin Cholesterol Noninvasive Detection Diffuse Reflectance Spectroscopy Atherosclerosis |
| DOI | 10.3964/j.issn.1000-0593(2016)10-3215-07 |
| 文献子类 | Article |
| 英文摘要 | Due to the urgent need for noninvasive detection of skin cholesterol, a portable, intelligent and real-time skin diffuse reflectance spectroscopy measurement system was designed based on a micro-spectrometer. Digitonin-horseradish peroxidase copolymer solution was prepared. According to the properties digitonin binds to the hydroxy of cholesterol molecular specifically and the horseradish peroxidase reacts with TMB color solution (the main component is 3,3',5,5'-tetramethylbenzidine) a color change was produced, by which the skin cholesterol was identified and instructed with high sensitivity and high specificity, and the concentration of skin cholesterol was quantified by measuring the degree of color change. In order to validate the feasibility of this method, pig skin which is similar to human skin was taken as the experimental subject, and cholesterol samples of gradient concentration were achieved through the extraction. After that the spectroscopy measurement system was adopted to detect the cholesterol concentration. The experiment result showed that, relative diffuse reflectance can distinguish the cholesterol samples with different concentrations, and the diffuse reflectance intensity factor can quantity the concentrations of cholesterol at characteristic wavelengths (442, 450 and 463 nm) and characteristic wavelength band of 442 similar to 500 nm. Linear fitting curves were obtained with the determination coefficient R-2 were 0. 960, 0. 959, 0. 958 and 0. 958, respectively. The study has shown that, using diffuse reflectance spectroscopy technology can realize noninvasive rapid detection of skin cholesterol, and applying it to the risk assessment of atherosclerotic diseases would contribute to the prevention and control of such diseases significantly. |
| WOS关键词 | YOUNG |
| WOS研究方向 | Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000385475500025 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/30125]  |
| 专题 | 合肥物质科学研究院_应用技术研究所 |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Anhui Prov Engn Technol Res Ctr Biomed Opt Instru, Wanjiang Ctr Dev Emerging Ind Technol, Tongling 244000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou Hua-yi,Dong Mei-li,Wang Yi-kun,et al. Rapid and Noninvasive Detection of Skin Cholesterol with Diffuse Reflectance Spectroscopy Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2016,36(10):3215-3221. |
| APA | Hou Hua-yi,Dong Mei-li,Wang Yi-kun,Zhu Ling,Ma Zu-chang,&Liu Yong.(2016).Rapid and Noninvasive Detection of Skin Cholesterol with Diffuse Reflectance Spectroscopy Technology.SPECTROSCOPY AND SPECTRAL ANALYSIS,36(10),3215-3221. |
| MLA | Hou Hua-yi,et al."Rapid and Noninvasive Detection of Skin Cholesterol with Diffuse Reflectance Spectroscopy Technology".SPECTROSCOPY AND SPECTRAL ANALYSIS 36.10(2016):3215-3221. |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。