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Rapid and Low-Cost CRP Measurement by Integrating a Paper-Based Microfluidic Immunoassay with Smartphone (CRP-Chip)

文献类型：期刊论文

作者Dong, Meili1,2; Wu, Jiandong2; Ma, Zimin2; Peretz-Soroka, Hagit2; Zhang, Michael3; Komenda, Paul3; Tangri, Navdeep3; Liu, Yong1; Rigatto, Claudio3; Lin, Francis2,4,5,6
刊名SENSORS
出版日期2017-04-01
卷号17期号:4
关键词C-reactive Protein Protein Biomarkers Chronic Disease Diagnosis Microfluidic Device Smartphone Low-cost
DOI10.3390/s17040684
文献子类Article
英文摘要Traditional diagnostic tests for chronic diseases are expensive and require a specialized laboratory, therefore limiting their use for point-of-care (PoC) testing. To address this gap, we developed a method for rapid and low-cost C-reactive protein (CRP) detection from blood by integrating a paper-based microfluidic immunoassay with a smartphone (CRP-Chip). We chose CRP for this initial development because it is a strong biomarker of prognosis in chronic heart and kidney disease. The microfluidic immunoassay is realized by lateral flow and gold nanoparticle-based colorimetric detection of the target protein. The test image signal is acquired and analyzed using a commercial smartphone with an attached microlens and a 3D-printed chip-phone interface. The CRP-Chip was validated for detecting CRP in blood samples from chronic kidney disease patients and healthy subjects. The linear detection range of the CRP-Chip is up to 2 mu g/mL and the detection limit is 54 ng/mL. The CRP-Chip test result yields high reproducibility and is consistent with the standard ELISA kit. A single CRP-Chip can perform the test in triplicate on a single chip within 15 min for less than 50 US cents of material cost. This CRP-Chip with attractive features of low-cost, fast test speed, and integrated easy operation with smartphones has the potential to enable future clinical PoC chronic disease diagnosis and risk stratification by parallel measurements of a panel of protein biomarkers.
WOS关键词C-REACTIVE PROTEIN ; CHRONIC KIDNEY-DISEASE ; GLOMERULAR-FILTRATION-RATE ; CORONARY-HEART-DISEASE ; CARDIOVASCULAR MORTALITY ; ALL-CAUSE ; DEVICES ; RISK ; BURDEN ; ASSOCIATION
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:000400822900025
资助机构Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; NSERC ; NSERC ; NSERC ; NSERC ; NSERC ; NSERC ; NSERC ; NSERC ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Collaborative Health Research Project (CHRP) grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Canadian Institutes of Health Research (CIHR) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Chinese Academy of Sciences (CAS) ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; Mitacs ; NSERC ; NSERC ; NSERC ; NSERC ; NSERC ; NSERC ; NSERC ; NSERC ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; Winnipeg Rh Institute Foundation ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba ; University of Manitoba
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/33431]  
专题合肥物质科学研究院_应用技术研究所
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol, Hefei 230031, Peoples R China
2.Univ Manitoba, Dept Phys & Astron, Winnipeg, MB R3T 2N2, Canada
3.Seven Oaks Gen Hosp, Winnipeg, MB R2V 3M3, Canada
4.Univ Manitoba, Dept Biosyst Engn, Winnipeg, MB R3T 2N2, Canada
5.Univ Manitoba, Dept Immunol, Winnipeg, MB R3T 2N2, Canada
6.Univ Manitoba, Dept Biol Sci, Winnipeg, MB R3T 2N2, Canada
推荐引用方式
GB/T 7714		 Dong, Meili,Wu, Jiandong,Ma, Zimin,et al. Rapid and Low-Cost CRP Measurement by Integrating a Paper-Based Microfluidic Immunoassay with Smartphone (CRP-Chip)[J]. SENSORS,2017,17(4).
APA Dong, Meili.,Wu, Jiandong.,Ma, Zimin.,Peretz-Soroka, Hagit.,Zhang, Michael.,...&Lin, Francis.(2017).Rapid and Low-Cost CRP Measurement by Integrating a Paper-Based Microfluidic Immunoassay with Smartphone (CRP-Chip).SENSORS,17(4).
MLA Dong, Meili,et al."Rapid and Low-Cost CRP Measurement by Integrating a Paper-Based Microfluidic Immunoassay with Smartphone (CRP-Chip)".SENSORS 17.4(2017).

入库方式： OAI收割

来源：合肥物质科学研究院

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