Single shot, three dimensional fluorescence microscopy with a spatially rotating point spread function
文献类型:期刊论文
作者 | Wang, Zhaojun1,2; Cai, Yanan1,2; Liang, Yansheng1,2; Zhou, Xing1,2; Yan, Shaohui1; Dan, Dan1; Bianco, Piero R.3; Lei, Ming1; Yao, Baoli1 |
刊名 | BIOMEDICAL OPTICS EXPRESS |
出版日期 | 2017-12-01 |
卷号 | 8期号:12页码:5493-5506 |
ISSN号 | 2156-7085 |
DOI | 10.1364/BOE.8.005493 |
产权排序 | 1 |
文献子类 | Article |
英文摘要 | A wide-field fluorescence microscope with a double-helix point spread function (PSF) is constructed to obtain the specimen's three-dimensional distribution with a single snapshot. Spiral-phase-based computer-generated holograms (CGHs) are adopted to make the depth-of-field of the microscope adjustable. The impact of system aberrations on the double-helix PSF at high numerical aperture is analyzed to reveal the necessity of the aberration correction. A modified cepstrum-based reconstruction scheme is promoted in accordance with properties of the new double-helix PSF. The extended depth-of-field images and the corresponding depth maps for both a simulated sample and a tilted section slice of bovine pulmonary artery endothelial (BPAE) cells are recovered, respectively, verifying that the depth-of-field is properly extended and the depth of the specimen can be estimated at a precision of 23.4mn. This three-dimensional fluorescence microscope with a framerate-rank time resolution is suitable for studying the fast developing process of thin and sparsely distributed micron-scale cells in extended depth-of-field. (C) 2017 Optical Society of America under the terms of the USA Open Access Publishing Agreement |
WOS关键词 | EXTENDED DEPTH ; PHASE MASK ; FIELD ; LIGHT ; IMAGE ; FOCUS |
WOS研究方向 | Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
语种 | 英语 |
WOS记录号 | WOS:000417583800012 |
资助机构 | Natural Science Foundation of China (NSFC)(61522511 ; National Institutes of Health(GM100156) ; Natural Science Basic Research Plan in Shaanxi Province of China(2016JZ020) ; 81427802 ; 11474352 ; 61377008) |
源URL | [http://ir.opt.ac.cn/handle/181661/29444] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, shaanxi, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Buffalo, Dept Microbiol & Immunol, 12 Capen Hall, Buffalo, NY 14214 USA |
推荐引用方式 GB/T 7714 | Wang, Zhaojun,Cai, Yanan,Liang, Yansheng,et al. Single shot, three dimensional fluorescence microscopy with a spatially rotating point spread function[J]. BIOMEDICAL OPTICS EXPRESS,2017,8(12):5493-5506. |
APA | Wang, Zhaojun.,Cai, Yanan.,Liang, Yansheng.,Zhou, Xing.,Yan, Shaohui.,...&Yao, Baoli.(2017).Single shot, three dimensional fluorescence microscopy with a spatially rotating point spread function.BIOMEDICAL OPTICS EXPRESS,8(12),5493-5506. |
MLA | Wang, Zhaojun,et al."Single shot, three dimensional fluorescence microscopy with a spatially rotating point spread function".BIOMEDICAL OPTICS EXPRESS 8.12(2017):5493-5506. |
入库方式: OAI收割
来源:西安光学精密机械研究所
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