Qualitative and quantitative measurements of complex flows demand for fast single-shot fluorescence lifetime imaging (FLI) technology with high precision. A method, single-shot time-gated fluorescence lifetime imaging using three-frame images (TFI-TGFLI), is presented. To our knowledge, it is the first work to combine a three-gate rapid lifetime determination (RLD) scheme and a four-channel framing camera to achieve this goal. Di erent from previously proposed two-gate RLD schemes, TFI-TGFLI can provide a wider lifetime range 0.6 ∼ 13ns with reasonable precision. The performances of the proposed approach have been examined by both Monte-Carlo simulations and toluene seeded gas mixing jet diagnosis experiments. The measured average lifetimes of the whole excited areas agree well with the results obtained by the streak camera, and they are 7.6ns (N2= 7L/min; O22= 19L/min; O2= 1L/min) with the standard deviations of 1.7ns and 0.8ns among the lifetime image pixels, respectively. The concentration distributions of the quenchers and fluorescent species were further analyzed, and they are consistent with the experimental settings. © 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.