Utilization of deep-sea microbial esterase PHE21 to generate chiral sec-butyl acetate through kinetic resolutions
文献类型：期刊论文
| 作者 | Sun, AJ; Wang, YL; Xu, YK; Zhang, Y; Hu, YF |
| 刊名 | CHIRALITY
 |
| 出版日期 | 2018 |
| 卷号 | 30期号:8页码:1027-1035 |
| 通讯作者 | yunfeng.hu@scsio.ac.cn |
| 英文摘要 | We previously identified and characterized 1 novel deep-sea microbial esterase PHE21 and used PHE21 as a green biocatalyst to generate chiral ethyl (S)-3-hydroxybutyrate, 1 key chiral chemical, with high enantiomeric excess and yield through kinetic resolution. Herein, we further explored the potential of esterase PHE21 in the enantioselective preparation of secondary butanol, which was hard to be resolved by lipases/esterases. Despite the fact that chiral secondary butanols and their ester derivatives were hard to prepare, esterase PHE21 was used as a green biocatalyst in the generation of (S)-sec-butyl acetate through hydrolytic reactions and the enantiomeric excess, and the conversion of (S)-sec-butyl acetate reached 98% and 52%, respectively, after process optimization. Esterase PHE21 was also used to generate (R)-sec-butyl acetate through asymmetric transesterification reactions, and the enantiomeric excess and conversion of (R)-sec-butyl acetate reached 64% and 43%, respectively, after process optimization. Deep-sea microbial esterase PHE21 was characterized to be a useful biocatalyst in the kinetic resolution of secondary butanol and other valuable chiral secondary alcohols. |
| 学科主题 | Pharmacology & Pharmacy; Chemistry |
| 关键词[WOS] | biocatalysis ; chiral sec-butyl acetate ; deep-sea microorganism ; kinetic resolution ; novel microbial esterase |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16917]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Sun, AJ,Wang, YL,Xu, YK,et al. Utilization of deep-sea microbial esterase PHE21 to generate chiral sec-butyl acetate through kinetic resolutions[J]. CHIRALITY,2018,30(8):1027-1035. |
| APA | Sun, AJ,Wang, YL,Xu, YK,Zhang, Y,&Hu, YF.(2018).Utilization of deep-sea microbial esterase PHE21 to generate chiral sec-butyl acetate through kinetic resolutions.CHIRALITY,30(8),1027-1035. |
| MLA | Sun, AJ,et al."Utilization of deep-sea microbial esterase PHE21 to generate chiral sec-butyl acetate through kinetic resolutions".CHIRALITY 30.8(2018):1027-1035. |
入库方式： OAI收割
来源：南海海洋研究所
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