Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate
文献类型：期刊论文
| 作者 | Hu, YF; Wang, YL; Xu, YK; Zhang, Y; Sun, AJ |
| 刊名 | CHIRALITY
 |
| 出版日期 | 2018 |
| 卷号 | 30期号:6页码:769-776 |
| 通讯作者 | yunfeng.hu@scsio.ac.cn |
| 英文摘要 | The two enantiomers of ethyl 3-hydroxybutyrate are important intermediates for the synthesis of a great variety of valuable chiral drugs. The preparation of chiral drug intermediates through kinetic resolution reactions catalyzed by esterases/lipases has been demonstrated to be an efficient and environmentally friendly method. We previously functionally characterized microbial esterase PHE21 and used PHE21 as a biocatalyst to generate optically pure ethyl (S)-3-hydroxybutyrate. Herein, we also functionally characterized one novel salt-tolerant microbial esterase WDEst17 from the genome of Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085. Esterase WDEst17 was further developed as an efficient biocatalyst to generate (R)-3-hydroxybutyrate, an important chiral drug intermediate, with the enantiomeric excess being 99% and the conversion rate being 65.05%, respectively, after process optimization. Notably, the enantio-selectivity of esterase WDEst17 was opposite than that of esterase PHE21. The identification of esterases WDEst17 and PHE21 through genome mining of microorganisms provides useful biocatalysts for the preparation of valuable chiral drug intermediates. |
| 学科主题 | Pharmacology & Pharmacy; Chemistry |
| 关键词[WOS] | ethyl (R)-3-hydroxybutyrate ; kinetic resolution ; novel esterase ; opposite enantio-selectivity ; process optimization |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16935]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Hu, YF,Wang, YL,Xu, YK,et al. Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate[J]. CHIRALITY,2018,30(6):769-776. |
| APA | Hu, YF,Wang, YL,Xu, YK,Zhang, Y,&Sun, AJ.(2018).Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate.CHIRALITY,30(6),769-776. |
| MLA | Hu, YF,et al."Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate".CHIRALITY 30.6(2018):769-776. |
入库方式： OAI收割
来源：南海海洋研究所
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