Proteome and Transcriptome Analysis of Ovary, Intersex Gonads, and Testis Reveals Potential Key Sex Reversal/Differentiation Genes and Mechanism in Scallop Chlamys nobilis
文献类型：期刊论文
| 作者 | Shi, Y; Liu, WG; He, MX |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 20期号:2页码:220-245 |
| 通讯作者 | hmx2@scsio.ac.cn |
| 英文摘要 | Bivalve mollusks exhibit hermaphroditism and sex reversal/differentiation. Studies generally focus on transcriptional profiling and specific genes related to sex determination and differentiation. Few studies on sex reversal/differentiation have been reported. A combination analysis of gonad proteomics and transcriptomics was conducted on Chlamys nobilis to provide a systematic understanding of sex reversal/differentiation in bivalves. We obtained 4258 unique peptides and 93,731 unigenes with good correlation between messenger RNA and protein levels. Candidate genes in sex reversal/differentiation were found: 15 genes differentially expressed between sexes were identified and 12 had obvious sexual functions. Three novel genes (foxl2, beta-catenin, and sry) were expressed highly in intersex individuals and were likely involved in the control of gonadal sex in C. nobilis. High expression of foxl2 or beta-catenin may inhibit sry and activate 5-HT receptor and vitellogenin to maintain female development. High expression of sry may inhibit foxl2 and beta-catenin and activate dmrt2, fem-1, sfp2, sa6, Amy-1, APCP4, and PLK to maintain male function. High expression of sry, foxl2, and beta-catenin in C. nobilis may be involved in promoting and maintaining sex reversal/differentiation. The downstream regulator may not be dimorphic expressed genes, but genes expressed in intersex individuals, males and females. Different expression patterns of sex-related genes and gonadal histological characteristics suggested that C. nobilis may change its sex from male to female. These findings suggest highly conserved sex reversal/differentiation with diverged regulatory pathways during C. nobilis evolution. This study provides valuable genetic resources for understanding sex reversal/differentiation (intersex) mechanisms and pathways underlying bivalve reproductive regulation. |
| 学科主题 | Biotechnology & Applied Microbiology; Marine & Freshwater Biology |
| 关键词[WOS] | Proteome ; Transcriptome ; Sex reversal/differentiation ; Intersex individual ; Chlamys nobilis |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16974]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Shi, Y,Liu, WG,He, MX. Proteome and Transcriptome Analysis of Ovary, Intersex Gonads, and Testis Reveals Potential Key Sex Reversal/Differentiation Genes and Mechanism in Scallop Chlamys nobilis[J]. MARINE BIOTECHNOLOGY,2018,20(2):220-245. |
| APA | Shi, Y,Liu, WG,&He, MX.(2018).Proteome and Transcriptome Analysis of Ovary, Intersex Gonads, and Testis Reveals Potential Key Sex Reversal/Differentiation Genes and Mechanism in Scallop Chlamys nobilis.MARINE BIOTECHNOLOGY,20(2),220-245. |
| MLA | Shi, Y,et al."Proteome and Transcriptome Analysis of Ovary, Intersex Gonads, and Testis Reveals Potential Key Sex Reversal/Differentiation Genes and Mechanism in Scallop Chlamys nobilis".MARINE BIOTECHNOLOGY 20.2(2018):220-245. |
入库方式： OAI收割
来源：南海海洋研究所
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