Involvement of eIF2 alpha of Epinephelus coioides in the fish immune response to virus infection
文献类型：期刊论文
| 作者 | Zang, SQ; Zhang, X; Zhang, JC; Li, C; Wei, JG; Qin, QW |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 75页码:365-373 |
| 通讯作者 | weijg@scau.edu.cn ; qinqw@scau.edu.cn |
| 英文摘要 | The eukaryotic initiation factor 2 alpha subunit (eIF2 alpha) is a key translation regulator that plays an important role in different cellular pressures and stimuli, including virus infection. In the present study, an eIF2 alpha homolog (EcelF2 alpha) from the orange-spotted grouper (Epinephelus coioides) was cloned and its roles during fish viral infection were characterized. EcelF2 alpha encodes a putative protein of 315 amino acid residues, and shares a high degree of similarity with elF2 alpha s from other species. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that EcelF2 alpha was distributed in all examined tissues. Both of the expression levels of EceIF2 alpha in the spleen and head kidney of E. coioides were up-regulated when challenged with polyinosine-polycytidylic acid (poly[I:C]). EceIF2 alpha was abundantly distributed in both the cytoplasm and nucleus in grouper spleen (GS) cells. Over-expression of EceIF2 alpha improved the expression of red-spotted grouper nervous necrosis virus (RGNNV) genes in GS cells. In addition, EceIF2 alpha depressed the activation of NK-kappa B and IFN-beta. Furthermore, dephosphorylation inhibitor treatment led to a significant decrease of RGNNV gene transcription. Taken together, these results suggest that EcelF2 alpha might be involved in the fish immune response to virus challenge. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 关键词[WOS] | elF2 alpha ; Phosphorylation ; Epinephelus coioides ; RGNNV |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16976]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Zang, SQ,Zhang, X,Zhang, JC,et al. Involvement of eIF2 alpha of Epinephelus coioides in the fish immune response to virus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2018,75:365-373. |
| APA | Zang, SQ,Zhang, X,Zhang, JC,Li, C,Wei, JG,&Qin, QW.(2018).Involvement of eIF2 alpha of Epinephelus coioides in the fish immune response to virus infection.FISH & SHELLFISH IMMUNOLOGY,75,365-373. |
| MLA | Zang, SQ,et al."Involvement of eIF2 alpha of Epinephelus coioides in the fish immune response to virus infection".FISH & SHELLFISH IMMUNOLOGY 75(2018):365-373. |
入库方式： OAI收割
来源：南海海洋研究所
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