Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate
文献类型：期刊论文
| 作者 | Gong, YH; Ma, SM; Wang, YF; Xu, YK; Sun, AJ; Zhang, Y; Hu, YF |
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 36期号:2页码:473-482 |
| 通讯作者 | yunfeng.hu@scsio.ac.cn |
| 英文摘要 | A novel esterase EstC10 from Bacillus sp. CX01 isolated from the deep sea of the Western Pacific Ocean and the functionalities of EstC10 was characterized. At present, the reports about the kinetic resolution of racemic methyl 2-chloropropionate were quite rare. So we developed deep-sea microbial esterase EstC10 as a novel biocatalyst in the kinetic resolution of racemic methyl 2-chloropropionate and generate (R)-methyl 2-chloropropionate with high enantiomeric excess (> 99%) after the optimization of process parameters such as pH, temperature, organic co-solvents, surfactants, substrate concentration and reaction time. Notably, the optimal substrate concentration (80 mmol/L) of esterase EstC10 was higher than the kinetic resolution of another esterase, Est12-7 (50 mmol/L). The novel microbial esterase EstC10 identified from the deep sea was a promising green biocatalyst in the generation of (R)-methyl 2-chloropropionate as well of many other valuable chiral chemicals in industry. |
| 学科主题 | Marine & Freshwater Biology; Oceanography |
| 关键词[WOS] | biocatalysis ; deep-sea microorganisms ; novel esterase ; kinetic resolution ; (R)-methyl 2-chloropropionate |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16986]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Gong, YH,Ma, SM,Wang, YF,et al. Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(2):473-482. |
| APA | Gong, YH.,Ma, SM.,Wang, YF.,Xu, YK.,Sun, AJ.,...&Hu, YF.(2018).Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(2),473-482. |
| MLA | Gong, YH,et al."Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.2(2018):473-482. |
入库方式： OAI收割
来源：南海海洋研究所
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