Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara
文献类型：期刊论文
| 作者 | Liu, XF; Wu, YH; Wei, SN; Wang, N; Li, YZ; Zhang, NW; Li, PF; Qin, QW; Chen, SL |
| 刊名 | JOURNAL OF FISH BIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 92期号:2页码:298-307 |
| 通讯作者 | chensl@ysfri.ac.cn |
| 英文摘要 | A new brain-cell line, EMB, was developed from kelp grouper Epinephelus moara, a cultured marine fish. The EMB cells were subcultured for more than 60 passages. The cells were cultured in Leibovitz's L-15 medium (L15) supplemented with antibiotics, foetal bovine serum (FBS), 2-mercaptoethanol (2-ME) and basic fibroblast growth factor (bFGF). The cells could grow at 18-30 degrees C, with the maximum growth between 24 and 30 degrees C. The optimum FBS concentration for the cells growth ranged between 15 and 20%. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 45. After being transfected with pEGFP-N3 plasmid, the cells could successfully express green fluorescence protein (GFP), implying that this cell line can be used for transgenic studies. A significant cytopathic effect (CPE) was observed in the cells after infection with Singapore grouper iridovirus (SGIV) or red spotted grouper nervous necrosis virus (RGNNV) and the viral replication was confirmed by quantitative real-time PCR (qrt-PCR) assay, which suggested EMB's application potential for studies of SGIV and RGNNV. |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 关键词[WOS] | brain-cell line ; GFP ; LCDV ; NNV ; primary culture ; SGIV |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17008]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, XF,Wu, YH,Wei, SN,et al. Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara[J]. JOURNAL OF FISH BIOLOGY,2018,92(2):298-307. |
| APA | Liu, XF.,Wu, YH.,Wei, SN.,Wang, N.,Li, YZ.,...&Chen, SL.(2018).Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara.JOURNAL OF FISH BIOLOGY,92(2),298-307. |
| MLA | Liu, XF,et al."Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara".JOURNAL OF FISH BIOLOGY 92.2(2018):298-307. |
入库方式： OAI收割
来源：南海海洋研究所
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