The first tropical sea cucumber caspase-8 from Holothuria leucospilota: Molecular characterization, involvement of apoptosis and inducible expression by immune challenge
文献类型：期刊论文
| 作者 | Yan, AF; Ren, CH; Chen, T; Jiang, X; Sun, HY; Huo, D; Hu, CQ; Wen, J |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 72页码:124-131 |
| 通讯作者 | yanaifen@mail3.sysu.edu.cn ; rosemary166@sina.com ; chan1010@scsio.ac.cn ; jiangxiao4367@126.com ; hongyanlucky@scau.edu.cn ; 827920154@qq.com ; hucq@scsio.ac.cn ; jw82123@126.com |
| 英文摘要 | In this study, the first tropical sea cucumber caspase-8 named HLcaspase-8 was identified from Holothuria leucospilota. The full-length cDNA of HLcaspase-8 is 2293 bp in size, containing a 245 bp 5'-untranslated region (UTR), a 521 bp 3'-UTR and a 1527 bp open reading frame (ORF) encoding a protein of 508 amino acids with a deduced molecular weight of 57.47 kDa. Besides the common signatures of caspase family including conserved cysteine active site pentapeptide motif QACQG, P20 domain and P10 domain, HLcaspase-8 also contains a characteristic DED domain. The over-expression of HLcaspase-8 in HEK293T cells showed that HLcaspase-8 protein could induce apoptosis and the apoptosis could be promoted by TNF-alpha, indicating that the apoptosis induced by HLcaspase-8 might also be triggered via a receptor-mediated pathway. Moreover, the expression of HLcaspase-8 in in vitro experiments performed in coelomocytes was significantly up-regulated by lipopolysaccharides (LPS) or polyriboinosinic-polyribocytidylic Acid [poly (I:C)] challenge, suggesting that the sea cucumber caspase-8 might play some important roles in the innate immune defense against bacterial and viral infections. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 关键词[WOS] | Caspase-8 ; Holothuria leucospilota ; Apoptosis ; Innate immunity |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17032]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Yan, AF,Ren, CH,Chen, T,et al. The first tropical sea cucumber caspase-8 from Holothuria leucospilota: Molecular characterization, involvement of apoptosis and inducible expression by immune challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:124-131. |
| APA | Yan, AF.,Ren, CH.,Chen, T.,Jiang, X.,Sun, HY.,...&Wen, J.(2018).The first tropical sea cucumber caspase-8 from Holothuria leucospilota: Molecular characterization, involvement of apoptosis and inducible expression by immune challenge.FISH & SHELLFISH IMMUNOLOGY,72,124-131. |
| MLA | Yan, AF,et al."The first tropical sea cucumber caspase-8 from Holothuria leucospilota: Molecular characterization, involvement of apoptosis and inducible expression by immune challenge".FISH & SHELLFISH IMMUNOLOGY 72(2018):124-131. |
入库方式： OAI收割
来源：南海海洋研究所
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