Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51
文献类型：期刊论文
| 作者 | Huang, XC; Chen, C; Ren, CH; Li, YY; Deng, YQ; Yang, YY; Ding, XQ |
| 刊名 | BIOFOULING
 |
| 出版日期 | 2018 |
| 卷号 | 34期号:1页码:1-14 |
| 通讯作者 | chen.chang@scsio.ac.cn |
| 英文摘要 | Colanic acid (CA) is a group I extracellular polysaccharide (EPS) that contributes to resistance against adverse environments in many members of the Enterobacteriaceae. In the present study, a genetic locus EPSC putatively involved in CA biosynthesis was identified in Vibrio alginolyticus ZJ-51, which undergoes colony morphology variation between translucent/smooth (ZJ-T) and opaque/rugose (ZJ-O). EPSC in ZJ-T carries 21 ORFs and resembles the CA cluster of Escherichia coli K-12. The deletion of EPSC led to decreased EPS and biofilm formation in both genetic backgrounds but no alternation of lipopolysaccharide. The loss of this locus also changed the colony morphology of ZJ-O on the 2216E plate and reduced the motility of ZJ-T. Compared with ZJ-T, ZJ-O lacks a 10-kb fragment (eps(T)) in EPSC containing homologs of wecA, wzx and wzy that are essential for O-antigen synthesis. However, the deletion or overexpression of eps(T) resulted in no change of colony morphology, biofilm formation or EPS production. This study reported at the first time a genetic locus EPSC that may be involved in colanic acid synthesis in V. alginolyticus ZJ-51, and found that it was related to EPS biosynthesis, biofilm formation, colony morphology and motility, which may shed light on the environmental adaptation of the vibrios. |
| 学科主题 | Biotechnology & Applied Microbiology; Marine & Freshwater Biology |
| 关键词[WOS] | Colanic acid ; EPS ; biofilm formation ; colony phase variation ; Vibrio alginolyticus |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17034]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Huang, XC,Chen, C,Ren, CH,et al. Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51[J]. BIOFOULING,2018,34(1):1-14. |
| APA | Huang, XC.,Chen, C.,Ren, CH.,Li, YY.,Deng, YQ.,...&Ding, XQ.(2018).Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51.BIOFOULING,34(1),1-14. |
| MLA | Huang, XC,et al."Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51".BIOFOULING 34.1(2018):1-14. |
入库方式： OAI收割
来源：南海海洋研究所
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