A molluscan TNF receptor-associated factor 2 (TRAF2) was involved in host defense against immune challenges
文献类型：期刊论文
| 作者 | Qu, FF; Xiang, ZM; Zhou, YL; Qin, YP |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 71页码:105-115 |
| 通讯作者 | qufufa@163.com |
| 英文摘要 | Tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) is a member of the TRAF superfamily that acted as a key signal transduction protein and has been implicated in inflammatory and apoptosis processes in mammals. However, identification of TRAF2s in invertebrates is very limited and its function, in particular that under immune challenges, is still unknown. In this report, a molluscan TRAF2 gene (referred to as AwTRAF2) was cloned and characterized from the freshwater bivalve, Anodonta woodiana. The open reading frame (ORF) of AwTRAF2 was 1683 bp in length, which encoded a putative 560 amino acid-protein. The deduced AwTRAF2 sequence shared similar structural characteristics and close evolutionary relationship with mollusk TRAF2s. The tissue-specific expression analysis revealed that AwTRAF2 mRNA was broadly expressed in all tested tissues, with high expression in gill and hepatopancreas. In addition, in vivo injection experiments directly showed that AwTRAF2 mRNA levels in hepatopancreas were significantly up-regulated in response to bacterial pathogen (Vibrio alginolyticus and Staphylococcus aureus) and PAMPs (Lipopolysaccharides and Peptidoglycan) challenges. Moreover, fluorescence microscopy observations revealed that AwTRAF2 was mainly located in cytoplasm of HEK293T cells and its overexpression significantly increased the transcriptional activities of the NF-kappa B-Luc reporter gene in HEK293T cells. Taken together, this study provided the experimental evidence of the presence of a functional TRAF2 in freshwater bivalves, which revealed its involvement in host response to immune challenges in A. woodiana. (C) 2017 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 关键词[WOS] | TNF receptor-associated factor 2 ; Molecular characterization ; Immune challenge ; NF-kappa B ; Anodonta woodiana |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17041]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Qu, FF,Xiang, ZM,Zhou, YL,et al. A molluscan TNF receptor-associated factor 2 (TRAF2) was involved in host defense against immune challenges[J]. FISH & SHELLFISH IMMUNOLOGY,2018,71:105-115. |
| APA | Qu, FF,Xiang, ZM,Zhou, YL,&Qin, YP.(2018).A molluscan TNF receptor-associated factor 2 (TRAF2) was involved in host defense against immune challenges.FISH & SHELLFISH IMMUNOLOGY,71,105-115. |
| MLA | Qu, FF,et al."A molluscan TNF receptor-associated factor 2 (TRAF2) was involved in host defense against immune challenges".FISH & SHELLFISH IMMUNOLOGY 71(2018):105-115. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。