Expression and functional characterization of TRIF in orange-spotted grouper (Epinephelus coioides)
文献类型：期刊论文
| 作者 | Wei, JG; Zhang, X; Zang, SQ; Qin, QW |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 71页码:295-304 |
| 通讯作者 | qinqw@scau.edu.cn |
| 英文摘要 | Antiviral immune responses are triggered by the innate immune recognition of viral infection. Toll/interleukin-1 receptor (TIR) domain containing adapter inducing interferon-beta (TRIF) is an adapter in responding to activation of Toll-like receptors, which provides early clearance of viral pathogens. Our study focuses on the functional characterization of grouper TRIF (EcTRIF) based on the comparison of its sequence and functional evolution from grouper fish to mammals. The results show that the open reading frame of EcTRIF encoded a protein of 580 amino acids. Real-time PCR analysis indicates that EcTRIF was constitutively expressed in all the analyzed tissues in healthy grouper. EcTRIF was significantly induced in spleen post-LPS and poly (I:C) stimulation. Fluorescence microscopy shows that EcTRIF is colocalized with a Golgi apparatus marker, implying its unique subcellular localization in the Golgi apparatus. Luciferase reporter assays confirmed that EcTRIF was able to activate the IFN and NF-kappa B promoter. Overexpression of EcTRIF in grouper brain cells inhibited the replication of red-spotted grouper nervous necrosis virus (RGNNV). These results indicate that EcTRIF plays an important role in modulating antiviral innate immune responses. Our results have applications in functional studies on TRIF in teleost fish and immune evolution. (C) 2017 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 关键词[WOS] | Epinephelus coioides ; TRIF ; Cellular localization ; RGNNV ; Antiviral immunity |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17043]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Wei, JG,Zhang, X,Zang, SQ,et al. Expression and functional characterization of TRIF in orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2018,71:295-304. |
| APA | Wei, JG,Zhang, X,Zang, SQ,&Qin, QW.(2018).Expression and functional characterization of TRIF in orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,71,295-304. |
| MLA | Wei, JG,et al."Expression and functional characterization of TRIF in orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 71(2018):295-304. |
入库方式： OAI收割
来源：南海海洋研究所
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