Diisonitrile Natural Product SF2768 Functions As a Chalkophore That Mediates Copper Acquisition in Streptomyces thioluteus
文献类型：期刊论文
| 作者 | Wang, LJ; Zhu, MY; Zhang, QB; Zhang, X; Yang, PL; Liu, ZH; Deng, Y; Zhu, YG; Huang, XS; Han, L |
| 刊名 | ACS CHEMICAL BIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 12期号:12页码:3067-3075 |
| 通讯作者 | hejingjj@mail.hzau.edu.cn |
| 英文摘要 | A nonribosomal peptide synthetase (NRPS) gene duster (sfa) was identified in Streptomyces thioluteus to direct the biosynthesis of the diisonitrile antibiotic SF2768. Its biosynthetic pathway was reasonably proposed based on bioinformatics analysis, metabolic profiles of mutants, and the elucidation of the intermediate and shunt product structures. Bioinformatics-based alignment found a putative ATP-binding cassette (ABC) transporter related to iron import within the biosynthetic gene: cluster, which implied that the product might be a siderophore. However, characterization of the metal-binding properties by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), metal-ligand titration, thin-layer chromatography (TLC), and chrome azurol S (CAS) assays revealed that the final product SF2768 and its diisonitrile derivatives specifically bind :topper, rather than iron, to forth. stable complexes. Inductively coupled plasma mass spectrometry (ICP-MS) analysis revealed that the intracellular cupric content of S. thioluteus significantly increased upon incubation with the copper-SF2768 complex, direct evidence for the copper, acquisition function of SF2768. Further in vivo functional characterization of the transport elements for the copper-SF2768 complexes not only confirmed the chalkophore identity of the compound but also gave initial clues into the copper uptake mechanism of this nonmethanotrophic microorganism. |
| 学科主题 | Biochemistry & Molecular Biology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/17044]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, LJ,Zhu, MY,Zhang, QB,et al. Diisonitrile Natural Product SF2768 Functions As a Chalkophore That Mediates Copper Acquisition in Streptomyces thioluteus[J]. ACS CHEMICAL BIOLOGY,2018,12(12):3067-3075. |
| APA | Wang, LJ.,Zhu, MY.,Zhang, QB.,Zhang, X.,Yang, PL.,...&He, J.(2018).Diisonitrile Natural Product SF2768 Functions As a Chalkophore That Mediates Copper Acquisition in Streptomyces thioluteus.ACS CHEMICAL BIOLOGY,12(12),3067-3075. |
| MLA | Wang, LJ,et al."Diisonitrile Natural Product SF2768 Functions As a Chalkophore That Mediates Copper Acquisition in Streptomyces thioluteus".ACS CHEMICAL BIOLOGY 12.12(2018):3067-3075. |
入库方式： OAI收割
来源：南海海洋研究所
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