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Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8

文献类型：期刊论文

作者Min, Jun1; Chen, Weiwei1; Wang, Jinpei2; Hu, Xiaoke1; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
刊名FRONTIERS IN MICROBIOLOGY
出版日期2017-09-13
卷号8
关键词2-chloro-5-nitrophenol Catabolism Cupriavidus Sp Strain Cnp-8 Degradation Kinetics Molecular Mechanism
ISSN号1664-302X
DOI10.3389/fmicb.2017.01778
产权排序第1完成单位
文献子类Article
英文摘要Compound 2-chloro-5-nitrophenol (2C5NP) is a typical chlorinated nitroaromatic pollutant. To date, the bacteria with the ability to degrade 2C5NP are rare, and the molecular mechanism of 2C5NP degradation remains unknown. In this study, Cupriavidus sp. strain CNP-8 utilizing 2-chloro-5-nitrophenol (2C5NP) and meta-nitrophenol (MNP) via partial reductive pathways was isolated from pesticide-contaminated soil. Biodegradation kinetic analysis indicated that 2C5NP degradation by this strain was concentration dependent, with a maximum specific degradation rate of 21.2 +/- 2.3 mu M h(-1). Transcriptional analysis showed that the mnp genes are up-regulated in both 2C5NP- and MNP-induced strain CNP-8. Two Mnp proteins were purified to homogeneity by Ni-NTA affinity chromatography. In addition to catalyzing the reduction of MNP, MnpA, a NADPH-dependent nitroreductase, also catalyzes the partial reduction of 2C5NP to 2-chloro-5-hydroxylaminophenol via 2-chloro-5-nitrosophenol, which was firstly identified as an intermediate of 2C5NP catabolism. MnpC, an aminohydroquinone dioxygenase, is likely responsible for the ring-cleavage reaction of 2C5NP degradation. Gene knockout and complementation indicated that mnpA is necessary for both 2C5NP and MNP catabolism. To our knowledge, strain CNP-8 is the second 2C5NP-utilizing bacterium, and this is the first report of the molecular mechanism of microbial 2C5NP degradation.
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WOS关键词RALSTONIA-EUTROPHA JMP134 ; PSEUDOMONAS SP JHN ; PARA-NITROPHENOL ; 2-CHLORO-4-NITROPHENOL DEGRADATION ; SPHINGOMONAS-PAUCIMOBILIS ; CLEAVAGE DIOXYGENASE ; AEROBIC DEGRADATION ; CATABOLISM PATHWAY ; DIETHYL PHTHALATE ; CONTAMINATED SOIL
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000410482100001
资助机构National Natural Science Foundation of China(31600085) ; National Key Research Program of China(2016YFC1402300) ; Foreword Key Priority Research Program of Chinese Academy of Sciences(QYZDB-SSW-DQC013) ; State Key Laboratory of Microbial Metabolism, Shanghai Tong University(MMLKF15-04)
源URL[http://ir.yic.ac.cn/handle/133337/23422]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者Hu, Xiaoke; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China)
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Min, Jun,Chen, Weiwei,Wang, Jinpei,et al. Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8[J]. FRONTIERS IN MICROBIOLOGY,2017,8.
APA Min, Jun,Chen, Weiwei,Wang, Jinpei,Hu, Xiaoke,&Hu, Xiaoke.(2017).Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8.FRONTIERS IN MICROBIOLOGY,8.
MLA Min, Jun,et al."Genetic and Biochemical Characterization of 2-Chloro-5-Nitrophenol Degradation in a Newly Isolated Bacterium, Cupriavidus sp Strain CNP-8".FRONTIERS IN MICROBIOLOGY 8(2017).

入库方式： OAI收割

来源：烟台海岸带研究所

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